Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 91413..91939 Replicon plasmid unnamed
Accession NZ_CP033743
Organism Citrobacter freundii strain FDAARGOS_549

Toxin (Protein)


Gene name relE Uniprot ID A0A855ELU0
Locus tag EGX89_RS00505 Protein ID WP_032611731.1
Coordinates 91652..91939 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EGX89_RS00500 Protein ID WP_000534858.1
Coordinates 91413..91652 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX89_RS00485 88226..89107 + 882 WP_123924671.1 hypothetical protein -
EGX89_RS00490 89331..90116 - 786 WP_123924672.1 hypothetical protein -
EGX89_RS00495 90549..91244 + 696 WP_123924673.1 DJ-1/PfpI family protein -
EGX89_RS00500 91413..91652 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EGX89_RS00505 91652..91939 + 288 WP_032611731.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGX89_RS00510 92010..92168 + 159 WP_040231232.1 type I toxin-antitoxin system Hok family toxin -
EGX89_RS00520 93071..93427 + 357 WP_123924674.1 hypothetical protein -
EGX89_RS00525 93470..94288 + 819 WP_087879592.1 N-6 DNA methylase -
EGX89_RS00530 94368..94859 + 492 WP_048227660.1 antirestriction protein -
EGX89_RS00535 94904..95227 + 324 WP_123924675.1 DUF957 domain-containing protein -
EGX89_RS00540 95190..95447 - 258 WP_040231225.1 helix-turn-helix transcriptional regulator -
EGX89_RS00550 96202..96348 + 147 WP_048227658.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..127175 127175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11148.22 Da        Isoelectric Point: 10.3898

>T114202 WP_032611731.1 NZ_CP033743:91652-91939 [Citrobacter freundii]
MAYLLDFDERALKEWRKLGSTVREQLKKKLAEVLESPRIEVNKLRGMPDCYKIKLKSSGYRLVYQVIDEKVVVFVVSVGK
RERSKVYSEAVKRIL

Download         Length: 288 bp

>T114202 NZ_CP033743:91652-91939 [Citrobacter freundii]
ATGGCGTATTTACTGGATTTTGACGAGCGCGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGCTGAAGTACTTGAGTCACCCCGAATTGAAGTGAATAAGCTTCGTGGTATGCCTGACTGTTACAAGATAA
AGCTCAAGTCTTCGGGTTATCGCCTTGTATATCAGGTCATAGACGAGAAGGTTGTCGTCTTCGTGGTTTCTGTTGGGAAA
AGAGAACGCTCGAAAGTGTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT114202 WP_000534858.1 NZ_CP033743:91413-91652 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT114202 NZ_CP033743:91413-91652 [Citrobacter freundii]
ATGGGTAGCATAAACCTGCGTATTGATGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTTACTCC
CTCTGAAGCGCTTCGTCTCATGCTTGAATATATCGCTGACAATGAACGTTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGACGCTGAACTCGTGGAGATAGTGAAAGAGCGTCTTCGCAATCCTAAGCCAGTACGTGTGACGCTGGATGAGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855ELU0

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References