Detailed information of TA system TA114201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 67620..68257
• Replicon: plasmid unnamed
• Accession: NZ_CP033743
• Organism: Citrobacter freundii strain FDAARGOS_549

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: EGX89_RS00355
• Protein ID: WP_032662169.1
• Coordinates: 67620..68030 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: EGX89_RS00360
• Protein ID: WP_032662167.1
• Coordinates: 68027..68257 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX89_RS00330	62985..63335	+	351	WP_032662182.1	DUF305 domain-containing protein	-
EGX89_RS00335	63521..64429	-	909	WP_032662179.1	HNH endonuclease	-
EGX89_RS00345	64977..65999	-	1023	WP_123924651.1	DNA helicase UvrD	-
EGX89_RS00350	65984..67546	-	1563	WP_040119797.1	AAA family ATPase	-
EGX89_RS00355	67620..68030	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
EGX89_RS00360	68027..68257	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EGX89_RS00370	69114..69419	+	306	WP_123924652.1	hypothetical protein	-
EGX89_RS00375	69506..70114	+	609	WP_123924653.1	hypothetical protein	-
EGX89_RS00380	70173..70952	+	780	WP_123924654.1	site-specific integrase	-
EGX89_RS00385	71237..71446	+	210	WP_123924655.1	hypothetical protein	-
EGX89_RS00390	71926..72237	-	312	WP_123924656.1	hypothetical protein	-
EGX89_RS00395	72624..72917	-	294	WP_123924657.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..127175	127175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T114201 WP_032662169.1 NZ_CP033743:c68030-67620 [Citrobacter freundii]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T114201 NZ_CP033743:c68030-67620 [Citrobacter freundii]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT114201 WP_032662167.1 NZ_CP033743:c68257-68027 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT114201 NZ_CP033743:c68257-68027 [Citrobacter freundii]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA