114200

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3853181..3853801 Replicon chromosome
Accession NZ_CP033742
Organism Citrobacter freundii strain FDAARGOS_550

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EGY10_RS18725 Protein ID WP_002892050.1
Coordinates 3853181..3853399 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag EGY10_RS18730 Protein ID WP_003021733.1
Coordinates 3853427..3853801 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY10_RS18690 3848539..3849168 + 630 WP_003835929.1 membrane protein -
EGY10_RS18695 3849233..3849586 + 354 WP_003831033.1 DUF1428 family protein -
EGY10_RS18700 3849638..3851191 - 1554 WP_121539795.1 EAL domain-containing protein -
EGY10_RS18705 3851380..3851640 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
EGY10_RS18710 3851642..3851782 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
EGY10_RS18715 3851861..3852331 - 471 WP_003021724.1 YlaC family protein -
EGY10_RS18720 3852448..3852999 - 552 WP_121539796.1 maltose O-acetyltransferase -
EGY10_RS18725 3853181..3853399 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EGY10_RS18730 3853427..3853801 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
EGY10_RS18735 3854289..3857438 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
EGY10_RS18740 3857461..3858654 - 1194 WP_088902653.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114200 WP_002892050.1 NZ_CP033742:c3853399-3853181 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T114200 NZ_CP033742:c3853399-3853181 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT114200 WP_003021733.1 NZ_CP033742:c3853801-3853427 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT114200 NZ_CP033742:c3853801-3853427 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References