Detailed information of TA system TA114199

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3202164..3202867
• Replicon: chromosome
• Accession: NZ_CP033742
• Organism: Citrobacter freundii strain FDAARGOS_550

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EGY10_RS15700
• Protein ID: WP_089586935.1
• Coordinates: 3202526..3202867 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EGY10_RS15695
• Protein ID: WP_058647031.1
• Coordinates: 3202164..3202505 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY10_RS15660	3197315..3198190	+	876	WP_058647036.1	GTPase family protein	-
EGY10_RS15665	3198434..3199150	+	717	WP_058647035.1	WYL domain-containing protein	-
EGY10_RS15670	3199186..3199638	+	453	WP_089586936.1	hypothetical protein	-
EGY10_RS15675	3199710..3200183	+	474	WP_058647034.1	hypothetical protein	-
EGY10_RS15680	3200303..3201124	+	822	WP_058647033.1	DUF945 domain-containing protein	-
EGY10_RS15685	3201155..3201598	+	444	WP_058647032.1	antirestriction protein	-
EGY10_RS15690	3201611..3202153	+	543	WP_080395938.1	DNA repair protein RadC	-
EGY10_RS15695	3202164..3202505	+	342	WP_058647031.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGY10_RS15700	3202526..3202867	+	342	WP_089586935.1	TA system toxin CbtA family protein	Toxin
EGY10_RS15705	3203233..3204105	-	873	WP_121540721.1	HNH endonuclease	-
EGY10_RS15710	3204246..3204881	+	636	WP_121540722.1	HNH endonuclease	-
EGY10_RS15715	3204928..3205290	-	363	WP_048219798.1	endoribonuclease SymE	-
EGY10_RS15720	3206069..3207439	+	1371	WP_003837154.1	tyrosine phenol-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12677.63 Da
• Isoelectric Point: 8.0323

>T114199 WP_089586935.1 NZ_CP033742:3202526-3202867 [Citrobacter freundii]
MKTLPAITPQAATLCLSPVAVWQMLLTRLLEQHYGLTLNDTQFSDETVIQEHIDAGITLANAVNFLVVKYELVRIDRKGL
SWQEQSPYLRAVDILRARQATGLLRRNSSNSGL

Nucleotide

• Length: 342 bp

>T114199 NZ_CP033742:3202526-3202867 [Citrobacter freundii]
ATGAAAACTCTACCTGCAATAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCTGTGGCAGTCTGGCAAATGTTACTGAC
ACGCCTGTTGGAACAGCACTATGGTCTTACACTAAACGATACGCAGTTCAGTGATGAAACGGTTATACAGGAACATATCG
ATGCCGGTATTACTCTGGCTAATGCCGTTAACTTTTTGGTGGTGAAATATGAATTGGTTCGTATCGATCGCAAAGGACTT
AGCTGGCAAGAGCAGTCCCCATATCTTCGGGCTGTAGATATTCTCCGGGCACGACAGGCAACTGGCTTGTTGCGACGAAA
CAGTAGTAACTCCGGATTGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12820.44 Da
• Isoelectric Point: 5.5353

>AT114199 WP_058647031.1 NZ_CP033742:3202164-3202505 [Citrobacter freundii]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLYYLADRASITGEFNNAESLKLDEVLPHFISQMESMLTTGEMNPRH
AHCVTLYHNCFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 342 bp

>AT114199 NZ_CP033742:3202164-3202505 [Citrobacter freundii]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTAAAATGGGGACTGAATCGTAATGTGACACCTTGCTTTGGTGCTCG
TCTAGTACAGGAGGGAAACCGGTTGTACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAATAATGCAGAATCTT
TGAAGTTGGATGAAGTACTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAACCCTCGCCAT
GCCCACTGTGTCACTCTGTACCACAATTGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGC
CATTTATCCCACACAACGCTAA