Detailed information of TA system TA114197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2701512..2702128
• Replicon: chromosome
• Accession: NZ_CP033742
• Organism: Citrobacter freundii strain FDAARGOS_550

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EGY10_RS13250
• Protein ID: WP_003840448.1
• Coordinates: 2701754..2702128 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B5NTK4
• Locus tag: EGY10_RS13245
• Protein ID: WP_043018956.1
• Coordinates: 2701512..2701754 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY10_RS13215	2697362..2697961	+	600	WP_016151258.1	glucose-1-phosphatase	-
EGY10_RS13220	2697955..2698827	+	873	WP_003840437.1	virulence factor BrkB family protein	-
EGY10_RS13225	2698824..2699261	+	438	WP_003840439.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EGY10_RS13230	2699306..2700247	+	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
EGY10_RS13240	2700398..2701306	-	909	WP_032950680.1	alpha/beta hydrolase	-
EGY10_RS13245	2701512..2701754	+	243	WP_043018956.1	ribbon-helix-helix domain-containing protein	Antitoxin
EGY10_RS13250	2701754..2702128	+	375	WP_003840448.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGY10_RS13255	2702165..2703094	-	930	WP_003840450.1	formate dehydrogenase accessory protein FdhE	-
EGY10_RS13260	2703091..2703726	-	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
EGY10_RS13265	2703723..2704625	-	903	WP_003028691.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13658.89 Da
• Isoelectric Point: 9.6923

>T114197 WP_003840448.1 NZ_CP033742:2701754-2702128 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEFGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Nucleotide

• Length: 375 bp

>T114197 NZ_CP033742:2701754-2702128 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATTTGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9451.75 Da
• Isoelectric Point: 4.3437

>AT114197 WP_043018956.1 NZ_CP033742:2701512-2701754 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT114197 NZ_CP033742:2701512-2701754 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6B5NTK4