Detailed information of TA system TA114195

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1657647..1658301
• Replicon: chromosome
• Accession: NZ_CP033742
• Organism: Citrobacter freundii strain FDAARGOS_550

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A0J1NHY7
• Locus tag: EGY10_RS08180
• Protein ID: WP_003026936.1
• Coordinates: 1657647..1658054 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: EGY10_RS08185
• Protein ID: WP_121540428.1
• Coordinates: 1658035..1658301 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY10_RS08160	1653595..1655328	-	1734	WP_121540427.1	single-stranded-DNA-specific exonuclease RecJ	-
EGY10_RS08165	1655334..1656047	-	714	WP_003825520.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
EGY10_RS08170	1656071..1656967	-	897	WP_003026928.1	site-specific tyrosine recombinase XerD	-
EGY10_RS08175	1657081..1657602	+	522	WP_003026933.1	flavodoxin FldB	-
EGY10_RS08180	1657647..1658054	-	408	WP_003026936.1	protein YgfX	Toxin
EGY10_RS08185	1658035..1658301	-	267	WP_121540428.1	FAD assembly factor SdhE	Antitoxin
EGY10_RS08190	1658558..1659538	+	981	WP_003838269.1	tRNA-modifying protein YgfZ	-
EGY10_RS08195	1659632..1660291	-	660	WP_003838267.1	hemolysin III family protein	-
EGY10_RS08200	1660454..1660765	-	312	WP_003026949.1	N(4)-acetylcytidine aminohydrolase	-
EGY10_RS08205	1660818..1661546	+	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
EGY10_RS08210	1661667..1663100	+	1434	WP_048218552.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15953.89 Da
• Isoelectric Point: 11.4054

>T114195 WP_003026936.1 NZ_CP033742:c1658054-1657647 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Nucleotide

• Length: 408 bp

>T114195 NZ_CP033742:c1658054-1657647 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10572.11 Da
• Isoelectric Point: 5.1700

>AT114195 WP_121540428.1 NZ_CP033742:c1658301-1658035 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT114195 NZ_CP033742:c1658301-1658035 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACACCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J1NHY7