Detailed information of TA system TA114186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 56707..57844
• Replicon: plasmid unnamed2
• Accession: NZ_CP033741
• Organism: Enterococcus sp. FDAARGOS_553

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EGX73_RS15820
• Protein ID: WP_123867122.1
• Coordinates: 56707..57570 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: EGX73_RS15825
• Protein ID: WP_000301765.1
• Coordinates: 57572..57844 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX73_RS15800	52757..53233	-	477	Protein_53	hypothetical protein	-
EGX73_RS15805	53568..53936	+	369	WP_123867120.1	metalloregulator ArsR/SmtB family transcription factor	-
EGX73_RS15810	53929..56058	+	2130	WP_115234514.1	cadmium-translocating P-type ATPase	-
EGX73_RS15815	56094..56555	+	462	WP_123867121.1	copper chaperone Copz family protein	-
EGX73_RS15820	56707..57570	-	864	WP_123867122.1	zeta toxin family protein	Toxin
EGX73_RS15825	57572..57844	-	273	WP_000301765.1	antitoxin	Antitoxin
EGX73_RS15830	57861..58070	-	210	WP_000527318.1	peptide-binding protein	-
EGX73_RS15835	58217..60361	-	2145	WP_123867123.1	type IA DNA topoisomerase	-
EGX73_RS15840	60400..60648	-	249	WP_070629349.1	hypothetical protein	-
EGX73_RS15845	60665..60877	-	213	WP_031644655.1	hypothetical protein	-
EGX73_RS15850	60899..61288	-	390	WP_056948698.1	hypothetical protein	-
EGX73_RS15855	61361..61963	-	603	WP_031644647.1	recombinase family protein	-
EGX73_RS16025	61977..62147	-	171	WP_164742224.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64163	64163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32497.13 Da
• Isoelectric Point: 7.3945

>T114186 WP_123867122.1 NZ_CP033741:c57570-56707 [Enterococcus sp. FDAARGOS_553]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTEVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPNNLETLKKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVASKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLRPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T114186 NZ_CP033741:c57570-56707 [Enterococcus sp. FDAARGOS_553]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGAAGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAGCAAGCACATGATATTGTTGTTAAAAATTTACCTAA
CAATTTAGAAACACTTAAAAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTAGCAAGTAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCGGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT114186 WP_000301765.1 NZ_CP033741:c57844-57572 [Enterococcus sp. FDAARGOS_553]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT114186 NZ_CP033741:c57844-57572 [Enterococcus sp. FDAARGOS_553]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3