Detailed information of TA system TA114179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1715675..1716204
• Replicon: chromosome
• Accession: NZ_CP033735
• Organism: Staphylococcus cohnii strain FDAARGOS_538

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EGX68_RS08815
• Protein ID: WP_103211251.1
• Coordinates: 1715675..1716037 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6N7Y6B0
• Locus tag: EGX68_RS08820
• Protein ID: WP_019468009.1
• Coordinates: 1716034..1716204 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX68_RS08795	1712707..1713477	-	771	WP_019468014.1	RNA polymerase sigma factor SigB	-
EGX68_RS08800	1713452..1713934	-	483	WP_019468013.1	anti-sigma B factor RsbW	-
EGX68_RS08805	1713936..1714262	-	327	WP_019468012.1	anti-sigma factor antagonist	-
EGX68_RS08810	1714331..1715332	-	1002	WP_019468011.1	PP2C family protein-serine/threonine phosphatase	-
EGX68_RS08815	1715675..1716037	-	363	WP_103211251.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EGX68_RS08820	1716034..1716204	-	171	WP_019468009.1	hypothetical protein	Antitoxin
EGX68_RS08825	1716292..1717440	-	1149	WP_103211252.1	alanine racemase	-
EGX68_RS08830	1717546..1717896	-	351	WP_019468007.1	holo-ACP synthase	-
EGX68_RS08835	1717932..1718423	-	492	WP_019468006.1	PH domain-containing protein	-
EGX68_RS08840	1718416..1719921	-	1506	WP_019468005.1	PH domain-containing protein	-
EGX68_RS08845	1719914..1720393	-	480	WP_019468004.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13621.72 Da
• Isoelectric Point: 9.8654

>T114179 WP_103211251.1 NZ_CP033735:c1716037-1715675 [Staphylococcus cohnii]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEEKMQEVNNAIDISLGLHNIRNYRT

Nucleotide

• Length: 363 bp

>T114179 NZ_CP033735:c1716037-1715675 [Staphylococcus cohnii]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTGTCACCAGTACAGGGTTCTGAACAAGGGGGAATAAGACCCGTAGT
CATTATACAAAATGATACTGGTAACAAGTATAGTCCTACCGTAATTGTAGCTGCAATTACAGGTAGGATTAATAAGGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGCAAGTATAAACTCGACAAGGATTCTGTTATATTGTTAGAGCAAATACGG
ACTTTAGATAAAAATAGATTAAAAGAAAAGTTGACATATTTATCTGAAGAAAAAATGCAAGAAGTTAATAATGCTATAGA
TATTAGCCTTGGTTTACATAATATTAGAAATTATAGAACATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6359.02 Da
• Isoelectric Point: 4.0121

>AT114179 WP_019468009.1 NZ_CP033735:c1716204-1716034 [Staphylococcus cohnii]
MASFNQNRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYIISKNNNE

Nucleotide

• Length: 171 bp

>AT114179 NZ_CP033735:c1716204-1716034 [Staphylococcus cohnii]
ATGGCAAGTTTTAATCAAAACAGAAATCATAGTATTGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGACTTGAA
TCTCTCCCTTGCGGCCGAAGCATTTCCATTTGAATGTGAAGCTTGCGATTGCAATGAAGTCTACATTATATCTAAGAATA
ACAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6N7Y6B0