Detailed information of TA system TA114173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 5412957..5413666
• Replicon: chromosome
• Accession: NZ_CP033730
• Organism: Achromobacter denitrificans strain PheN1

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: EC609_RS25595
• Protein ID: WP_123787472.1
• Coordinates: 5413283..5413666 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: EC609_RS25590
• Protein ID: WP_096665073.1
• Coordinates: 5412957..5413286 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC609_RS25565	5408765..5410060	-	1296	WP_123787468.1	site-specific integrase	-
EC609_RS25570	5410212..5410502	+	291	WP_123787469.1	hypothetical protein	-
EC609_RS25575	5410615..5411786	+	1172	WP_123787470.1	IS3 family transposase	-
EC609_RS25580	5411868..5412482	-	615	WP_123787471.1	NAD-dependent DNA ligase	-
EC609_RS25585	5412492..5412857	-	366	WP_096665075.1	hypothetical protein	-
EC609_RS25590	5412957..5413286	-	330	WP_096665073.1	helix-turn-helix domain-containing protein	Antitoxin
EC609_RS25595	5413283..5413666	-	384	WP_123787472.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EC609_RS25600	5413955..5415052	+	1098	WP_123787473.1	DNA-binding protein	-
EC609_RS25605	5415220..5415753	+	534	Protein_5046	thiamine biosynthesis protein ThiF	-
EC609_RS25610	5415741..5416217	+	477	WP_123787474.1	Mov34/MPN/PAD-1 family protein	-
EC609_RS25615	5416543..5417079	+	537	WP_123787475.1	DNA repair protein RadC	-
EC609_RS25620	5417348..5417695	+	348	WP_123787476.1	DUF2958 domain-containing protein	-
EC609_RS25625	5417733..5418404	-	672	WP_123788136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5408765..5480371	71606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14104.18 Da
• Isoelectric Point: 10.1013

>T114173 WP_123787472.1 NZ_CP033730:c5413666-5413283 [Achromobacter denitrificans]
MSKQARKPLKWVSSAKRDLDAMPDDVKDVFGHAIDLAQAGGKHQDAKVMTGFGSAGVLEVVEDHQGDTYRAVYTVKFAGW
VYVLHCFQKKSKSGIATPKPDMDLINIRLKAAKRDFEAWQAQQGARK

Nucleotide

• Length: 384 bp

>T114173 NZ_CP033730:c5413666-5413283 [Achromobacter denitrificans]
ATGTCGAAGCAAGCCCGTAAACCCCTCAAATGGGTCAGTTCCGCCAAGCGGGACCTCGATGCGATGCCCGACGATGTCAA
GGATGTTTTTGGCCATGCCATCGACCTGGCCCAGGCCGGCGGCAAGCACCAGGATGCCAAGGTCATGACGGGTTTCGGTT
CGGCCGGCGTGCTGGAGGTGGTCGAGGACCACCAGGGCGACACCTATCGGGCCGTCTACACCGTCAAGTTCGCCGGCTGG
GTCTATGTCCTGCACTGCTTCCAGAAGAAGTCTAAAAGCGGCATCGCGACGCCGAAACCGGACATGGATTTGATCAACAT
CCGCCTGAAGGCCGCGAAGCGGGATTTCGAAGCCTGGCAGGCACAGCAAGGAGCACGCAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12046.75 Da
• Isoelectric Point: 9.6890

>AT114173 WP_096665073.1 NZ_CP033730:c5413286-5412957 [Achromobacter denitrificans]
MNEHNEITIEEGSTNVYADLGYTDAAEMQRKSQLAGEIARAIKARRLTQEDAATLLGIDQSKVSRITRGQFRGVSEAKLL
ELVARLGHDVKIVVGPVRRRAGKIELQFA

Nucleotide

• Length: 330 bp

>AT114173 NZ_CP033730:c5413286-5412957 [Achromobacter denitrificans]
ATGAATGAGCACAACGAAATCACCATCGAAGAAGGCAGCACCAATGTCTACGCCGACCTGGGTTACACCGACGCAGCCGA
GATGCAGCGCAAGTCCCAGCTCGCCGGCGAGATTGCCCGTGCGATCAAGGCGCGCCGCCTGACGCAGGAAGACGCAGCTA
CGCTGCTGGGCATCGATCAGTCCAAGGTTTCACGTATCACCCGCGGCCAGTTCCGCGGCGTCAGCGAGGCCAAGCTGCTC
GAACTGGTGGCCCGGCTGGGCCACGACGTGAAGATCGTGGTCGGCCCCGTGCGGCGGCGCGCCGGCAAAATCGAGCTGCA
GTTTGCTTGA