Detailed information of TA system TA114172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1199741..1200783
• Replicon: chromosome
• Accession: NZ_CP033730
• Organism: Achromobacter denitrificans strain PheN1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EC609_RS05630
• Protein ID: WP_003109777.1
• Coordinates: 1200208..1200783 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: EC609_RS05625
• Protein ID: WP_003050245.1
• Coordinates: 1199741..1200211 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC609_RS05590	1195133..1196551	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
EC609_RS05595	1196541..1197452	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
EC609_RS05600	1197449..1198141	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
EC609_RS05605	1198138..1198536	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
EC609_RS05610	1198548..1198907	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
EC609_RS05615	1198924..1199157	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
EC609_RS05620	1199154..1199537	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
EC609_RS05625	1199741..1200211	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
EC609_RS05630	1200208..1200783	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
EC609_RS05635	1200801..1201715	+	915	WP_003105629.1	AAA family ATPase	-
EC609_RS05640	1201712..1202182	+	471	WP_003105626.1	hypothetical protein	-
EC609_RS05645	1202179..1202679	+	501	WP_003090159.1	hypothetical protein	-
EC609_RS05650	1202679..1203581	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
EC609_RS05655	1203620..1204345	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1139336..1246183	106847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T114172 WP_003109777.1 NZ_CP033730:1200208-1200783 [Achromobacter denitrificans]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T114172 NZ_CP033730:1200208-1200783 [Achromobacter denitrificans]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT114172 WP_003050245.1 NZ_CP033730:1199741-1200211 [Achromobacter denitrificans]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT114172 NZ_CP033730:1199741-1200211 [Achromobacter denitrificans]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68