Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2776562..2777082 Replicon chromosome
Accession NZ_CP033723
Organism Clavibacter michiganensis subsp. nebraskensis strain 61-1

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag EGX35_RS13100 Protein ID WP_015491236.1
Coordinates 2776562..2776852 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag EGX35_RS13105 Protein ID WP_041465310.1
Coordinates 2776849..2777082 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX35_RS13085 2774511..2776109 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
EGX35_RS13090 2776137..2776241 - 105 WP_080606018.1 hypothetical protein -
EGX35_RS13095 2776271..2776522 + 252 Protein_2570 helix-turn-helix transcriptional regulator -
EGX35_RS13100 2776562..2776852 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGX35_RS13105 2776849..2777082 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
EGX35_RS13110 2777194..2777394 - 201 WP_015491238.1 hypothetical protein -
EGX35_RS13115 2777410..2777637 - 228 WP_015491239.1 antitoxin VbhA family protein -
EGX35_RS13120 2777729..2778793 + 1065 WP_045929310.1 LD-carboxypeptidase -
EGX35_RS13125 2778970..2779329 + 360 WP_045929311.1 hypothetical protein -
EGX35_RS13130 2779526..2780005 + 480 WP_015491243.1 hypothetical protein -
EGX35_RS13135 2780152..2781189 + 1038 WP_045929312.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T114170 WP_015491236.1 NZ_CP033723:c2776852-2776562 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T114170 NZ_CP033723:c2776852-2776562 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT114170 WP_041465310.1 NZ_CP033723:c2777082-2776849 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT114170 NZ_CP033723:c2777082-2776849 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References