Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2776556..2777076 Replicon chromosome
Accession NZ_CP033722
Organism Clavibacter michiganensis subsp. nebraskensis strain 7580

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag EGX37_RS13105 Protein ID WP_015491236.1
Coordinates 2776556..2776846 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag EGX37_RS13110 Protein ID WP_041465310.1
Coordinates 2776843..2777076 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX37_RS13090 2774505..2776103 + 1599 WP_015491235.1 class I SAM-dependent methyltransferase -
EGX37_RS13095 2776131..2776235 - 105 WP_080606018.1 hypothetical protein -
EGX37_RS13100 2776265..2776516 + 252 Protein_2571 helix-turn-helix transcriptional regulator -
EGX37_RS13105 2776556..2776846 - 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGX37_RS13110 2776843..2777076 - 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
EGX37_RS13115 2777188..2777388 - 201 WP_015491238.1 hypothetical protein -
EGX37_RS13120 2777404..2777631 - 228 WP_015491239.1 antitoxin VbhA family protein -
EGX37_RS13125 2777723..2778787 + 1065 WP_045929310.1 LD-carboxypeptidase -
EGX37_RS13130 2778964..2779323 + 360 WP_045929311.1 hypothetical protein -
EGX37_RS13135 2779484..2779999 + 516 WP_041465311.1 hypothetical protein -
EGX37_RS13140 2780146..2781183 + 1038 WP_045929312.1 DUF1266 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T114169 WP_015491236.1 NZ_CP033722:c2776846-2776556 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T114169 NZ_CP033722:c2776846-2776556 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT114169 WP_041465310.1 NZ_CP033722:c2777076-2776843 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT114169 NZ_CP033722:c2777076-2776843 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References