Detailed information of TA system TA114165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1603037..1603657
• Replicon: chromosome
• Accession: NZ_CP033717
• Organism: Yersinia pseudotuberculosis strain FDAARGOS_579

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: EGX53_RS08050
• Protein ID: WP_002208622.1
• Coordinates: 1603454..1603657 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: Q66DR4
• Locus tag: EGX53_RS08045
• Protein ID: WP_002218472.1
• Coordinates: 1603037..1603405 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX53_RS08025	1600546..1601004	+	459	WP_002213775.1	IS200/IS605-like element IS1541B family transposase	-
EGX53_RS08030	1601242..1601502	+	261	WP_002208617.1	type B 50S ribosomal protein L31	-
EGX53_RS08035	1601518..1601661	+	144	WP_002208618.1	type B 50S ribosomal protein L36	-
EGX53_RS08040	1602525..1602878	+	354	WP_012105569.1	hypothetical protein	-
EGX53_RS08045	1603037..1603405	+	369	WP_002218472.1	Hha toxicity modulator TomB	Antitoxin
EGX53_RS08050	1603454..1603657	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
EGX53_RS08060	1604563..1604952	+	390	WP_024063215.1	MGMT family protein	-
EGX53_RS08065	1605088..1605609	-	522	WP_011191872.1	YbaY family lipoprotein	-
EGX53_RS08070	1605866..1606726	+	861	WP_002208625.1	acyl-CoA thioesterase II	-
EGX53_RS08075	1606987..1608276	-	1290	WP_002228344.1	ammonium transporter AmtB	-
EGX53_RS08080	1608316..1608654	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1600546..1601004	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T114165 WP_002208622.1 NZ_CP033717:1603454-1603657 [Yersinia pseudotuberculosis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T114165 NZ_CP033717:1603454-1603657 [Yersinia pseudotuberculosis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACTATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14215.00 Da
• Isoelectric Point: 4.6121

>AT114165 WP_002218472.1 NZ_CP033717:1603037-1603405 [Yersinia pseudotuberculosis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Nucleotide

• Length: 369 bp

>AT114165 NZ_CP033717:1603037-1603405 [Yersinia pseudotuberculosis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S

Antitoxin

• AlphaFold DB: Q66DR4