Detailed information of TA system TA114154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 14893..15427
• Replicon: plasmid unnamed
• Accession: NZ_CP033716
• Organism: Yersinia pseudotuberculosis strain FDAARGOS_579

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: EGX53_RS00120
• Protein ID: WP_002213360.1
• Coordinates: 15128..15427 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: EGX53_RS00115
• Protein ID: WP_002224342.1
• Coordinates: 14893..15135 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX53_RS00095	11459..12625	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
EGX53_RS00100	12622..13587	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
EGX53_RS22045	13747..14028	+	282	WP_164491551.1	hypothetical protein	-
EGX53_RS22050	14177..14350	-	174	WP_002213357.1	hypothetical protein	-
EGX53_RS00110	14435..14727	+	293	Protein_20	IS6 family transposase	-
EGX53_RS00115	14893..15135	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EGX53_RS00120	15128..15427	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX53_RS00125	15567..16226	-	660	WP_123767105.1	type III secretion system effector GTPase activator YopE	-
EGX53_RS00130	16420..16812	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
EGX53_RS00135	16875..17519	-	645	Protein_25	IS3 family transposase	-
EGX53_RS00140	17622..18794	+	1173	WP_011191364.1	IS21 family transposase	-
EGX53_RS00145	18794..19192	+	399	Protein_27	ATP-binding protein	-
EGX53_RS00150	19570..19995	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopB / yopD / yopM / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV	1..66641	66641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T114154 WP_002213360.1 NZ_CP033716:15128-15427 [Yersinia pseudotuberculosis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T114154 NZ_CP033716:15128-15427 [Yersinia pseudotuberculosis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT114154 WP_002224342.1 NZ_CP033716:14893-15135 [Yersinia pseudotuberculosis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT114154 NZ_CP033716:14893-15135 [Yersinia pseudotuberculosis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6