Detailed information of TA system TA114140

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2345613..2346419
• Replicon: chromosome
• Accession: NZ_CP033715
• Organism: Yersinia pseudotuberculosis strain FDAARGOS_580

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EGX52_RS11385
• Protein ID: WP_057532451.1
• Coordinates: 2346036..2346419 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3G5KEF9
• Locus tag: EGX52_RS11380
• Protein ID: WP_050090622.1
• Coordinates: 2345613..2345981 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX52_RS11340	2340642..2340860	-	219	WP_057532456.1	helix-turn-helix domain-containing protein	-
EGX52_RS11345	2340955..2341602	-	648	WP_057532447.1	inovirus Gp2 family protein	-
EGX52_RS11350	2342201..2343178	+	978	WP_123784591.1	hypothetical protein	-
EGX52_RS11355	2343254..2344087	+	834	WP_057532449.1	DUF945 domain-containing protein	-
EGX52_RS11360	2344099..2344322	+	224	Protein_2001	DUF905 domain-containing protein	-
EGX52_RS11365	2344397..2344867	+	471	WP_057532450.1	antirestriction protein	-
EGX52_RS11370	2344877..2345350	+	474	WP_050090624.1	DNA repair protein RadC	-
EGX52_RS11375	2345360..2345581	+	222	WP_050090623.1	DUF987 domain-containing protein	-
EGX52_RS11380	2345613..2345981	+	369	WP_050090622.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGX52_RS11385	2346036..2346419	+	384	WP_057532451.1	TA system toxin CbtA family protein	Toxin
EGX52_RS11390	2346416..2346907	+	492	WP_050090620.1	hypothetical protein	-
EGX52_RS23430	2346921..2347082	-	162	WP_155407244.1	hypothetical protein	-
EGX52_RS11395	2347081..2347929	+	849	WP_057532452.1	DUF4942 domain-containing protein	-
EGX52_RS11400	2348025..2348273	+	249	WP_050090618.1	ribbon-helix-helix domain-containing protein	-
EGX52_RS11405	2348277..2348573	+	297	WP_072080376.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EGX52_RS11410	2348911..2350146	+	1236	WP_072091827.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2332580..2348573	15993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-116)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14375.49 Da
• Isoelectric Point: 8.9747

>T114140 WP_057532451.1 NZ_CP033715:2346036-2346419 [Yersinia pseudotuberculosis]
MQTLPLTPQRAASSCPSPVEIWQKLLTHLLEQHYGLTLSDTPFSNETTIREHIDAGISLSDAVNFLVEKYELVRTDQPGF
TLMKQSPFITPIDILRARQATGLMKRRGYKAVSDITRGKASRMEQHH

Nucleotide

• Length: 384 bp

>T114140 NZ_CP033715:2346036-2346419 [Yersinia pseudotuberculosis]
ATGCAAACTTTACCTTTAACCCCGCAACGGGCGGCTTCGTCCTGCCCGTCACCTGTTGAAATATGGCAAAAACTGCTGAC
ACATCTGCTCGAACAACACTATGGTCTGACGCTCAGTGATACTCCATTCAGCAATGAAACCACCATTCGCGAACATATCG
ACGCGGGGATCTCCCTCAGCGATGCGGTGAACTTTCTGGTGGAGAAGTATGAACTGGTGCGCACTGACCAGCCTGGTTTC
ACCTTAATGAAGCAGTCACCGTTTATCACTCCAATCGATATTCTCCGCGCCAGGCAAGCCACCGGTCTGATGAAGCGCAG
GGGTTATAAAGCGGTGAGTGATATTACCCGTGGCAAAGCGAGTCGGATGGAGCAACACCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13494.34 Da
• Isoelectric Point: 5.5999

>AT114140 WP_050090622.1 NZ_CP033715:2345613-2345981 [Yersinia pseudotuberculosis]
MKSVALSGIPPEASPCQQWGLNREVTPVFGARLVQEGNRLHFLTDRAGFNGAFSEDDALRLDQAFPLMLKQLEIELRSGE
LNPRRQHCVTLNHNGLTCEADTLGSYGYVYIAIYPTSLCSPV

Nucleotide

• Length: 369 bp

>AT114140 NZ_CP033715:2345613-2345981 [Yersinia pseudotuberculosis]
ATGAAATCTGTCGCCCTGTCCGGTATACCACCGGAGGCCAGCCCCTGCCAGCAATGGGGATTGAATCGCGAAGTTACACC
CGTCTTTGGTGCGCGTCTGGTGCAGGAAGGCAATCGTCTGCATTTTCTGACTGATCGTGCTGGGTTTAATGGTGCATTCA
GTGAGGATGACGCGCTACGGCTCGACCAGGCATTTCCGCTGATGCTCAAACAGCTTGAAATTGAGCTGCGCTCAGGTGAG
CTCAATCCTCGTCGTCAACATTGCGTTACGTTGAACCACAATGGCCTGACCTGCGAAGCCGACACCCTCGGTTCTTACGG
GTATGTTTATATTGCCATTTACCCGACCAGCCTGTGCTCACCTGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G5KEF9