Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3151581..3152337 Replicon chromosome
Accession NZ_CP033711
Organism Yersinia pseudotuberculosis strain FDAARGOS_582

Toxin (Protein)


Gene name ypjF Uniprot ID A0A3G5KDU1
Locus tag EGX44_RS15385 Protein ID WP_032467110.1
Coordinates 3151969..3152337 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID Q664A5
Locus tag EGX44_RS15380 Protein ID WP_011193303.1
Coordinates 3151581..3151916 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX44_RS15345 3146720..3147499 + 780 WP_001297096.1 IS21-like element IS100kyp family helper ATPase IstB -
EGX44_RS15350 3147565..3147801 - 237 Protein_2743 inovirus Gp2 family protein -
EGX44_RS15355 3148466..3148759 + 294 Protein_2744 ATP-binding protein -
EGX44_RS15360 3148813..3149457 + 645 WP_080871705.1 hypothetical protein -
EGX44_RS15365 3149705..3150259 + 555 WP_011193306.1 hypothetical protein -
EGX44_RS15370 3150287..3150907 + 621 WP_011193305.1 hypothetical protein -
EGX44_RS15375 3151090..3151569 + 480 WP_011193304.1 hypothetical protein -
EGX44_RS15380 3151581..3151916 + 336 WP_011193303.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EGX44_RS15385 3151969..3152337 + 369 WP_032467110.1 TA system toxin CbtA family protein Toxin
EGX44_RS15390 3152564..3153418 + 855 WP_032467109.1 DUF4942 domain-containing protein -
EGX44_RS15395 3153793..3154065 + 273 WP_011193300.1 Arm DNA-binding domain-containing protein -
EGX44_RS15400 3154300..3154707 + 408 WP_011193299.1 hypothetical protein -
EGX44_RS15405 3154730..3155056 + 327 WP_011193298.1 hypothetical protein -
EGX44_RS15410 3154998..3155498 + 501 WP_002214364.1 virulence RhuM family protein -
EGX44_RS15415 3155495..3156253 - 759 WP_011193297.1 ABC transporter ATP-binding protein -
EGX44_RS15420 3156250..3157257 - 1008 WP_011193296.1 iron chelate uptake ABC transporter family permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3137701..3162814 25113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13723.81 Da        Isoelectric Point: 6.4530

>T114117 WP_032467110.1 NZ_CP033711:3151969-3152337 [Yersinia pseudotuberculosis]
MQILPVTPKRAAYACPSPVVVWQSMLTYLLEQHYGLALNDTEFSDDAVIHEYIDAGISLSDALNFTVEKFGLVRIDRRGF
SCQEQSPFITAIDILRARRATGLMTRLGYQTIISVIRGEKQT

Download         Length: 369 bp

>T114117 NZ_CP033711:3151969-3152337 [Yersinia pseudotuberculosis]
ATGCAGATCTTACCTGTTACTCCGAAACGGGCGGCTTATGCTTGCCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTGGAACAGCATTACGGATTGGCTCTTAACGATACCGAGTTCAGTGATGACGCCGTGATCCACGAATATATTG
ATGCTGGTATCTCTCTGTCCGATGCGTTGAATTTCACGGTGGAAAAGTTTGGCCTGGTTCGTATCGATCGCCGGGGGTTC
TCCTGCCAGGAACAATCTCCCTTCATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCCACCGGCCTGATGACCCGTCT
GGGTTACCAGACCATCATCTCAGTTATCCGGGGCGAAAAGCAGACATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12452.06 Da        Isoelectric Point: 6.0555

>AT114117 WP_011193303.1 NZ_CP033711:3151581-3151916 [Yersinia pseudotuberculosis]
MPSITTPAWGLKRNVTPQFGARLVQEGDRLHFLADRADINGTFSEVQVRDLDNAFPQFINYLELMLLSGELNPRHQHCVT
LYRNGLTCEADSLGSHGYVYLAIYPTPQSTA

Download         Length: 336 bp

>AT114117 NZ_CP033711:3151581-3151916 [Yersinia pseudotuberculosis]
ATGCCATCAATCACTACCCCAGCCTGGGGACTTAAACGCAACGTCACTCCGCAGTTTGGTGCCCGTCTGGTACAGGAAGG
CGACCGACTACATTTTCTAGCCGACCGTGCCGATATCAACGGAACCTTCAGCGAAGTGCAGGTACGTGATTTAGATAACG
CCTTCCCGCAGTTCATCAACTACCTGGAACTGATGCTGCTCTCCGGTGAGCTCAACCCTCGCCACCAGCACTGTGTCACG
TTGTATCGCAATGGCTTGACCTGTGAGGCCGATAGCCTGGGATCACACGGTTATGTGTATCTCGCTATTTATCCTACTCC
CCAATCAACTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G5KDU1

Antitoxin

Source ID Structure
AlphaFold DB Q664A5

References