Detailed information of TA system TA114075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2118028..2118469
• Replicon: chromosome
• Accession: NZ_CP033704
• Organism: Burkholderia pseudomallei strain FDAARGOS_593

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: EGY16_RS32380
• Protein ID: WP_009939402.1
• Coordinates: 2118296..2118469 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: EGY16_RS32375
• Protein ID: WP_004525420.1
• Coordinates: 2118028..2118312 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY16_RS32340	2113136..2114398	-	1263	WP_004554781.1	c-type cytochrome	-
EGY16_RS32345	2114557..2116017	-	1461	WP_038765619.1	metallophosphoesterase	-
EGY16_RS32350	2116264..2116380	+	117	Protein_1685	IS3 family transposase	-
EGY16_RS32355	2116607..2117035	-	429	Protein_1686	IS110 family transposase	-
EGY16_RS32360	2116998..2117381	-	384	Protein_1687	DDE-type integrase/transposase/recombinase	-
EGY16_RS32375	2118028..2118312	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
EGY16_RS32380	2118296..2118469	-	174	WP_009939402.1	BrnT family toxin	Toxin
EGY16_RS38540	2118700..2119158	+	459	Protein_1690	prohibitin family protein	-
EGY16_RS32385	2119156..2119530	+	375	Protein_1691	DDE-type integrase/transposase/recombinase	-
EGY16_RS32390	2119707..2121409	-	1703	Protein_1692	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2110913..2123697	12784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T114075 WP_009939402.1 NZ_CP033704:c2118469-2118296 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T114075 NZ_CP033704:c2118469-2118296 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT114075 WP_004525420.1 NZ_CP033704:c2118312-2118028 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT114075 NZ_CP033704:c2118312-2118028 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8