Detailed information of TA system TA114071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 3123200..3123641
• Replicon: chromosome
• Accession: NZ_CP033701
• Organism: Burkholderia pseudomallei strain FDAARGOS_594

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: EGY14_RS15685
• Protein ID: WP_009939402.1
• Coordinates: 3123468..3123641 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: EGY14_RS15680
• Protein ID: WP_004525420.1
• Coordinates: 3123200..3123484 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY14_RS15645	3118289..3119551	-	1263	WP_004530738.1	c-type cytochrome	-
EGY14_RS15650	3119710..3121170	-	1461	WP_004547073.1	metallophosphoesterase	-
EGY14_RS15655	3121436..3121552	+	117	Protein_2489	IS3 family transposase	-
EGY14_RS15660	3121779..3122207	-	429	Protein_2490	IS110 family transposase	-
EGY14_RS15665	3122170..3122553	-	384	Protein_2491	DDE-type integrase/transposase/recombinase	-
EGY14_RS15680	3123200..3123484	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
EGY14_RS15685	3123468..3123641	-	174	WP_009939402.1	BrnT family toxin	Toxin
EGY14_RS15690	3124309..3124584	+	276	WP_004537565.1	bacteriophage protein Gp49	-
EGY14_RS37705	3124864..3125040	+	177	WP_004552958.1	hypothetical protein	-
EGY14_RS15705	3125069..3125416	+	348	WP_009946438.1	hypothetical protein	-
EGY14_RS37710	3125464..3125682	+	219	WP_004549687.1	hypothetical protein	-
EGY14_RS15715	3125857..3126447	-	591	WP_009946442.1	hypothetical protein	-
EGY14_RS15720	3126697..3126846	+	150	WP_004537646.1	bacteriophage protein Gp44	-
EGY14_RS37715	3126843..3127616	+	774	Protein_2500	prohibitin family protein	-
EGY14_RS37720	3127614..3128042	+	429	Protein_2501	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3122170..3139317	17147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T114071 WP_009939402.1 NZ_CP033701:c3123641-3123468 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T114071 NZ_CP033701:c3123641-3123468 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT114071 WP_004525420.1 NZ_CP033701:c3123484-3123200 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT114071 NZ_CP033701:c3123484-3123200 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8