Detailed information of TA system TA114053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 49212..49746
• Replicon: plasmid unnamed1
• Accession: NZ_CP033697
• Organism: Yersinia pestis strain FDAARGOS_601

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: EGX46_RS00340
• Protein ID: WP_002213360.1
• Coordinates: 49447..49746 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: EGX46_RS00335
• Protein ID: WP_002224342.1
• Coordinates: 49212..49454 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX46_RS00315	45822..46988	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
EGX46_RS00320	46985..47950	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
EGX46_RS23905	48110..48346	+	237	WP_154020274.1	hypothetical protein	-
EGX46_RS24015	48495..48668	-	174	WP_002213357.1	hypothetical protein	-
EGX46_RS00330	48754..49046	+	293	Protein_63	IS6 family transposase	-
EGX46_RS00335	49212..49454	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EGX46_RS00340	49447..49746	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX46_RS00345	49886..50545	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
EGX46_RS00350	50739..51131	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
EGX46_RS00355	51194..51838	-	645	Protein_68	IS3 family transposase	-
EGX46_RS00360	51941..53113	+	1173	WP_002220893.1	IS21 family transposase	-
EGX46_RS00365	53113..53511	+	399	Protein_70	ATP-binding protein	-
EGX46_RS00370	53887..54312	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA	1..70300	70300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T114053 WP_002213360.1 NZ_CP033697:49447-49746 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T114053 NZ_CP033697:49447-49746 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT114053 WP_002224342.1 NZ_CP033697:49212-49454 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT114053 NZ_CP033697:49212-49454 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6