Detailed information of TA system TA114039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 11995..12529
• Replicon: plasmid unnamed2
• Accession: NZ_CP033695
• Organism: Yersinia pestis strain FDAARGOS_602

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: EGX42_RS00755
• Protein ID: WP_002213360.1
• Coordinates: 12230..12529 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: EGX42_RS00750
• Protein ID: WP_002224342.1
• Coordinates: 11995..12237 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX42_RS00730	8605..9771	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
EGX42_RS00735	9768..10733	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
EGX42_RS22850	10893..11129	+	237	WP_154020274.1	hypothetical protein	-
EGX42_RS22985	11278..11451	-	174	WP_002213357.1	hypothetical protein	-
EGX42_RS00745	11537..11829	+	293	Protein_16	IS6 family transposase	-
EGX42_RS00750	11995..12237	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EGX42_RS00755	12230..12529	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX42_RS00760	12669..13328	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
EGX42_RS00765	13522..13914	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
EGX42_RS00770	13977..14621	-	645	Protein_21	IS3 family transposase	-
EGX42_RS22810	14724..14825	+	102	Protein_22	IS21 family transposase	-
EGX42_RS00785	14908..15930	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
EGX42_RS00790	15927..16709	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA / yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD	1..70174	70174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T114039 WP_002213360.1 NZ_CP033695:12230-12529 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T114039 NZ_CP033695:12230-12529 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT114039 WP_002224342.1 NZ_CP033695:11995-12237 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT114039 NZ_CP033695:11995-12237 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6