Detailed information of TA system TA114025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 18029..18563
• Replicon: plasmid unnamed1
• Accession: NZ_CP033689
• Organism: Yersinia pestis strain FDAARGOS_603

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: EGX74_RS00135
• Protein ID: WP_002213360.1
• Coordinates: 18264..18563 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: EGX74_RS00130
• Protein ID: WP_002224342.1
• Coordinates: 18029..18271 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX74_RS00110	14639..15805	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
EGX74_RS00115	15802..16767	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
EGX74_RS23780	16927..17163	+	237	WP_154020274.1	hypothetical protein	-
EGX74_RS23905	17312..17485	-	174	WP_002213357.1	hypothetical protein	-
EGX74_RS00125	17571..17863	+	293	Protein_23	IS6 family transposase	-
EGX74_RS00130	18029..18271	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EGX74_RS00135	18264..18563	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX74_RS00140	18703..19362	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
EGX74_RS00145	19556..19948	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
EGX74_RS00150	20011..20655	-	645	Protein_28	IS3 family transposase	-
EGX74_RS00155	20758..21930	+	1173	WP_002220893.1	IS21 family transposase	-
EGX74_RS00160	21930..22328	+	399	Protein_30	ATP-binding protein	-
EGX74_RS00165	22705..23130	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA / yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG	1..68554	68554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T114025 WP_002213360.1 NZ_CP033689:18264-18563 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T114025 NZ_CP033689:18264-18563 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT114025 WP_002224342.1 NZ_CP033689:18029-18271 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT114025 NZ_CP033689:18029-18271 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6