Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 2102387..2103051 Replicon chromosome
Accession NZ_CP033688
Organism Mycobacterium avium subsp. paratuberculosis strain Telford

Toxin (Protein)


Gene name vapC Uniprot ID Q73YC9
Locus tag EGA31_RS10000 Protein ID WP_003872398.1
Coordinates 2102644..2103051 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q73YC8
Locus tag EGA31_RS09995 Protein ID WP_003872399.1
Coordinates 2102387..2102647 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGA31_RS09975 2097803..2098465 + 663 WP_126215982.1 TetR/AcrR family transcriptional regulator -
EGA31_RS09980 2098661..2100118 + 1458 WP_003872402.1 serine/threonine protein kinase -
EGA31_RS09985 2100320..2101588 - 1269 WP_003872401.1 cytochrome P450 -
EGA31_RS09990 2101754..2102206 + 453 WP_003872400.1 VOC family protein -
EGA31_RS09995 2102387..2102647 + 261 WP_003872399.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EGA31_RS10000 2102644..2103051 + 408 WP_003872398.1 type II toxin-antitoxin system VapC family toxin Toxin
EGA31_RS10005 2103198..2104763 - 1566 WP_003872397.1 serine/threonine protein kinase -
EGA31_RS10010 2105000..2105521 + 522 WP_003878215.1 carboxymuconolactone decarboxylase family protein -
EGA31_RS10015 2105518..2106063 + 546 WP_003878214.1 carboxymuconolactone decarboxylase family protein -
EGA31_RS10020 2106184..2106714 + 531 WP_003878213.1 TetR/AcrR family transcriptional regulator -
EGA31_RS10025 2106703..2107872 - 1170 WP_003872395.1 acyl-CoA dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14418.47 Da        Isoelectric Point: 4.5642

>T114021 WP_003872398.1 NZ_CP033688:2102644-2103051 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Download         Length: 408 bp

>T114021 NZ_CP033688:2102644-2103051 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9635.01 Da        Isoelectric Point: 11.7137

>AT114021 WP_003872399.1 NZ_CP033688:2102387-2102647 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT114021 NZ_CP033688:2102387-2102647 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L5MIC8

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MIC5

References