Detailed information of TA system TA114020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1217569..1218133
• Replicon: chromosome
• Accession: NZ_CP033688
• Organism: Mycobacterium avium subsp. paratuberculosis strain Telford

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: EGA31_RS06135
• Protein ID: WP_003875391.1
• Coordinates: 1217762..1218133 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: EGA31_RS06130
• Protein ID: WP_003875390.1
• Coordinates: 1217569..1217775 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA31_RS06110	1212822..1213820	+	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
EGA31_RS06115	1213844..1215025	-	1182	WP_003875387.1	hypothetical protein	-
EGA31_RS06120	1215183..1216256	+	1074	WP_009975391.1	redox-regulated ATPase YchF	-
EGA31_RS06125	1216456..1217400	+	945	WP_003875389.1	HNH endonuclease	-
EGA31_RS06130	1217569..1217775	+	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EGA31_RS06135	1217762..1218133	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGA31_RS06140	1218233..1218673	+	441	WP_003875392.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
EGA31_RS06145	1218729..1219004	+	276	WP_003878531.1	hypothetical protein	-
EGA31_RS06150	1219106..1219504	+	399	WP_003875394.1	VOC family protein	-
EGA31_RS06155	1219571..1219954	+	384	WP_003875395.1	hypothetical protein	-
EGA31_RS06160	1220024..1220377	+	354	WP_003875396.1	hypothetical protein	-
EGA31_RS06165	1220555..1221058	+	504	WP_010949693.1	glycoside hydrolase	-
EGA31_RS06170	1221471..1221795	+	325	Protein_1202	antibiotic biosynthesis monooxygenase	-
EGA31_RS06175	1221800..1222483	-	684	WP_003875399.1	hypothetical protein	-
EGA31_RS06180	1222601..1222798	+	198	WP_016706054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T114020 WP_003875391.1 NZ_CP033688:1217762-1218133 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T114020 NZ_CP033688:1217762-1218133 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGCTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT114020 WP_003875390.1 NZ_CP033688:1217569-1217775 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT114020 NZ_CP033688:1217569-1217775 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2