Detailed information of TA system TA114008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1306172..1306842
• Replicon: chromosome
• Accession: NZ_CP033685
• Organism: Pseudomonas aeruginosa strain H26023

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EGV94_RS06340
• Protein ID: WP_033868686.1
• Coordinates: 1306423..1306842 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EGV94_RS06335
• Protein ID: WP_033868685.1
• Coordinates: 1306172..1306426 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGV94_RS06315	1302106..1303104	-	999	WP_199798120.1	LuxR family transcriptional regulator	-
EGV94_RS06320	1303199..1303423	+	225	WP_009363662.1	AlpA family phage regulatory protein	-
EGV94_RS06325	1303426..1304304	+	879	WP_033868683.1	helicase RepA family protein	-
EGV94_RS06330	1304291..1305043	+	753	WP_033868684.1	hypothetical protein	-
EGV94_RS06335	1306172..1306426	+	255	WP_033868685.1	Arc family DNA-binding protein	Antitoxin
EGV94_RS06340	1306423..1306842	+	420	WP_033868686.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGV94_RS06345	1306999..1307769	+	771	WP_033868687.1	P-type conjugative transfer protein TrbJ	-
EGV94_RS32165	1307780..1307938	+	159	WP_153570825.1	hypothetical protein	-
EGV94_RS06350	1307940..1309388	+	1449	WP_003137886.1	P-type conjugative transfer protein TrbL	-
EGV94_RS06355	1309457..1309699	+	243	WP_003137888.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
EGV94_RS06360	1309711..1309995	+	285	WP_003137890.1	hypothetical protein	-
EGV94_RS06365	1309998..1310366	+	369	WP_033868688.1	conjugal transfer transcriptional regulator TraJ	-
EGV94_RS06370	1310405..1310863	-	459	WP_065426345.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1300539..1324908	24369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14883.08 Da
• Isoelectric Point: 5.1890

>T114008 WP_033868686.1 NZ_CP033685:1306423-1306842 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYVSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T114008 NZ_CP033685:1306423-1306842 [Pseudomonas aeruginosa]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAGCCCGAGCCCAACCCAGCCGTGCGAGCTTGGTTGAATGA
GCAAGTAGCGGAAACCCTCTACGTGTCCAGCGTGACGCTGGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCCCTGGACGGCTTGCTCGAACTGTTCGGAGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCCATTATGCCGAGCTGGCGGTGAAGGCCCGCACAGCCGGCAAGGGTTTTCCAACACCCGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTCGAAGCGGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.81 Da
• Isoelectric Point: 6.4760

>AT114008 WP_033868685.1 NZ_CP033685:1306172-1306426 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKAPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT114008 NZ_CP033685:1306172-1306426 [Pseudomonas aeruginosa]
ATGGCAATCCTGACAGTGCGGAATGTGCCCGATGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTGCGCGAGATTCTGGAAAGTGCAGTGAAGTCGGAAAAGCGCATCCGCATGGGCGACGCTCTGGCGG
AGCTTGGCCGCCGCGTAGGGCTGACAAACGATGATTTCGCAGTGTTGGACCAGGTGCGCGACAAGGCACCGGCAGAACCC
ATGAGGTTTGAATGA