Detailed information of TA system TA114003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5763069..5763709
• Replicon: chromosome
• Accession: NZ_CP033684
• Organism: Pseudomonas aeruginosa strain H26027

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EGV95_RS27870
• Protein ID: WP_003105740.1
• Coordinates: 5763069..5763479 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: EGV95_RS27875
• Protein ID: WP_003158175.1
• Coordinates: 5763479..5763709 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGV95_RS27825	5758911..5759108	+	198	WP_003105756.1	hypothetical protein	-
EGV95_RS27830	5759264..5759992	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
EGV95_RS27835	5759998..5760546	+	549	WP_003105753.1	DUF3158 family protein	-
EGV95_RS27840	5760593..5761432	+	840	WP_003105750.1	Rha family transcriptional regulator	-
EGV95_RS27845	5761462..5761950	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
EGV95_RS27850	5762058..5762303	+	246	WP_023102333.1	CrpP family protein	-
EGV95_RS27855	5762369..5762587	-	219	WP_003105747.1	hypothetical protein	-
EGV95_RS27865	5762787..5763047	-	261	WP_003105742.1	hypothetical protein	-
EGV95_RS27870	5763069..5763479	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EGV95_RS27875	5763479..5763709	-	231	WP_003158175.1	antitoxin	Antitoxin
EGV95_RS27880	5763965..5765884	+	1920	WP_004352838.1	type I DNA topoisomerase	-
EGV95_RS27885	5766192..5766401	+	210	WP_003105733.1	cold-shock protein	-
EGV95_RS27890	5766623..5768512	+	1890	WP_016851610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5743498..5837210	93712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T114003 WP_003105740.1 NZ_CP033684:c5763479-5763069 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T114003 NZ_CP033684:c5763479-5763069 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT114003 WP_003158175.1 NZ_CP033684:c5763709-5763479 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT114003 NZ_CP033684:c5763709-5763479 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2