Detailed information of TA system TA114000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2961506..2962548
• Replicon: chromosome
• Accession: NZ_CP033684
• Organism: Pseudomonas aeruginosa strain H26027

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EGV95_RS14515
• Protein ID: WP_003050248.1
• Coordinates: 2961973..2962548 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: EGV95_RS14510
• Protein ID: WP_003050245.1
• Coordinates: 2961506..2961976 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGV95_RS14475	2956897..2958315	-	1419	WP_023087297.1	TIGR03752 family integrating conjugative element protein	-
EGV95_RS14480	2958305..2959216	-	912	WP_023087298.1	TIGR03749 family integrating conjugative element protein	-
EGV95_RS14485	2959213..2959905	-	693	WP_023087299.1	TIGR03746 family integrating conjugative element protein	-
EGV95_RS14490	2959902..2960300	-	399	WP_124014392.1	TIGR03750 family conjugal transfer protein	-
EGV95_RS14495	2960313..2960672	-	360	WP_021195311.1	TIGR03745 family integrating conjugative element membrane protein	-
EGV95_RS14500	2960689..2960922	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
EGV95_RS14505	2960919..2961302	-	384	WP_023087300.1	RAQPRD family integrative conjugative element protein	-
EGV95_RS14510	2961506..2961976	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
EGV95_RS14515	2961973..2962548	+	576	WP_003050248.1	PIN domain-containing protein	Toxin
EGV95_RS14520	2962566..2963480	+	915	WP_023087301.1	AAA family ATPase	-
EGV95_RS14525	2963477..2963947	+	471	WP_023087302.1	hypothetical protein	-
EGV95_RS14530	2963944..2964444	+	501	WP_003050262.1	hypothetical protein	-
EGV95_RS14535	2964444..2965346	+	903	WP_023087303.1	nucleotidyltransferase domain-containing protein	-
EGV95_RS14540	2965383..2966108	+	726	WP_023087304.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2892820..3008036	115216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21630.76 Da
• Isoelectric Point: 5.6130

>T114000 WP_003050248.1 NZ_CP033684:2961973-2962548 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T114000 NZ_CP033684:2961973-2962548 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT114000 WP_003050245.1 NZ_CP033684:2961506-2961976 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT114000 NZ_CP033684:2961506-2961976 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCGTTGCGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68