Detailed information of TA system TA113996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 11257..11852
• Replicon: plasmid p4_BGR80S
• Accession: NZ_CP033675
• Organism: Burkholderia glumae strain BGR80S

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: EFP20_RS30795
• Protein ID: WP_012734107.1
• Coordinates: 11502..11852 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: EFP20_RS30790
• Protein ID: WP_012734108.1
• Coordinates: 11257..11508 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFP20_RS30755 (EFP20_30550)	6526..6714	+	189	WP_017432455.1	hypothetical protein	-
EFP20_RS30760 (EFP20_30555)	7466..7750	+	285	WP_017432347.1	hypothetical protein	-
EFP20_RS30765	7764..8006	+	243	WP_124837682.1	hypothetical protein	-
EFP20_RS30770 (EFP20_30565)	8003..8248	+	246	WP_127913916.1	hypothetical protein	-
EFP20_RS30775 (EFP20_30570)	8305..8646	+	342	WP_012732710.1	hypothetical protein	-
EFP20_RS30780 (EFP20_30575)	8649..10484	+	1836	WP_012732711.1	site-specific integrase	-
EFP20_RS30785 (EFP20_30580)	10587..10994	+	408	WP_230674618.1	hypothetical protein	-
EFP20_RS30790 (EFP20_30585)	11257..11508	+	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EFP20_RS30795 (EFP20_30590)	11502..11852	+	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
EFP20_RS30800 (EFP20_30595)	11911..12105	+	195	WP_124837685.1	hypothetical protein	-
EFP20_RS30805	12255..12476	+	222	WP_017433768.1	hypothetical protein	-
EFP20_RS30810 (EFP20_30600)	12699..13163	+	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
EFP20_RS30815 (EFP20_30605)	13477..15852	+	2376	WP_232252305.1	TonB-dependent receptor	-
EFP20_RS30820 (EFP20_30610)	15884..16630	+	747	WP_232252306.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80567	80567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T113996 WP_012734107.1 NZ_CP033675:11502-11852 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T113996 NZ_CP033675:11502-11852 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT113996 WP_012734108.1 NZ_CP033675:11257-11508 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT113996 NZ_CP033675:11257-11508 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5