Detailed information of TA system TA113992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 132993..133630
• Replicon: plasmid p2_BGR80S
• Accession: NZ_CP033671
• Organism: Burkholderia glumae strain BGR80S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: EFP20_RS00535
• Protein ID: WP_012735347.1
• Coordinates: 132993..133394 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: C5AP39
• Locus tag: EFP20_RS00540
• Protein ID: WP_012735348.1
• Coordinates: 133394..133630 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFP20_RS00505 (EFP20_00495)	129456..129725	+	270	WP_012735343.1	hypothetical protein	-
EFP20_RS00510 (EFP20_00500)	129885..130499	+	615	Protein_101	error-prone DNA polymerase	-
EFP20_RS00515 (EFP20_00505)	130515..130802	+	288	WP_012735344.1	hypothetical protein	-
EFP20_RS00520	131906..132100	-	195	WP_124837731.1	hypothetical protein	-
EFP20_RS00525 (EFP20_00515)	132155..132397	-	243	WP_012735345.1	hypothetical protein	-
EFP20_RS00530 (EFP20_00520)	132415..132744	-	330	WP_155297075.1	hypothetical protein	-
EFP20_RS00535 (EFP20_00525)	132993..133394	-	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
EFP20_RS00540 (EFP20_00530)	133394..133630	-	237	WP_012735348.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EFP20_RS00545 (EFP20_00540)	133967..134656	-	690	WP_257739423.1	hypothetical protein	-
EFP20_RS00550 (EFP20_00545)	134743..136569	-	1827	WP_012735349.1	site-specific integrase	-
EFP20_RS00555 (EFP20_00550)	136566..136946	-	381	WP_080569442.1	hypothetical protein	-
EFP20_RS00560 (EFP20_00555)	136949..137266	-	318	WP_050811486.1	hypothetical protein	-
EFP20_RS00565 (EFP20_00560)	137317..138405	-	1089	Protein_112	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141000	141000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T113992 WP_012735347.1 NZ_CP033671:c133394-132993 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T113992 NZ_CP033671:c133394-132993 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8983.13 Da
• Isoelectric Point: 6.3676

>AT113992 WP_012735348.1 NZ_CP033671:c133630-133394 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGM

Nucleotide

• Length: 237 bp

>AT113992 NZ_CP033671:c133630-133394 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Structures

Toxin

• AlphaFold DB: C5AP38

Antitoxin

• AlphaFold DB: C5AP39