Detailed information of TA system TA113990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 173780..174375
• Replicon: plasmid p1_BGR21S
• Accession: NZ_CP033668
• Organism: Burkholderia glumae strain BGR21S

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: EFP19_RS30485
• Protein ID: WP_012734107.1
• Coordinates: 173780..174130 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: EFP19_RS30490
• Protein ID: WP_012734108.1
• Coordinates: 174124..174375 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFP19_RS30460 (EFP19_30370)	169002..169748	-	747	WP_232252306.1	energy transducer TonB	-
EFP19_RS30465 (EFP19_30375)	169780..172155	-	2376	WP_232252305.1	TonB-dependent receptor	-
EFP19_RS30470 (EFP19_30380)	172469..172933	-	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
EFP19_RS30475	173156..173377	-	222	WP_017433768.1	hypothetical protein	-
EFP19_RS30480 (EFP19_30385)	173527..173721	-	195	WP_124837685.1	hypothetical protein	-
EFP19_RS30485 (EFP19_30390)	173780..174130	-	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
EFP19_RS30490 (EFP19_30395)	174124..174375	-	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EFP19_RS30495 (EFP19_30400)	174638..175045	-	408	WP_230674618.1	hypothetical protein	-
EFP19_RS30500 (EFP19_30405)	175148..176983	-	1836	WP_124837684.1	site-specific integrase	-
EFP19_RS30505 (EFP19_30410)	176986..177327	-	342	WP_012732710.1	hypothetical protein	-
EFP19_RS30510 (EFP19_30415)	177384..177629	-	246	WP_127913916.1	hypothetical protein	-
EFP19_RS30515	177626..177868	-	243	WP_124837682.1	hypothetical protein	-
EFP19_RS30520 (EFP19_30425)	177882..178166	-	285	WP_017432347.1	hypothetical protein	-
EFP19_RS30525 (EFP19_30430)	178573..179061	-	489	WP_012732893.1	GNAT family N-acetyltransferase	-
EFP19_RS30530 (EFP19_30435)	179058..179327	-	270	WP_012732894.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181288	181288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T113990 WP_012734107.1 NZ_CP033668:c174130-173780 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T113990 NZ_CP033668:c174130-173780 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT113990 WP_012734108.1 NZ_CP033668:c174375-174124 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT113990 NZ_CP033668:c174375-174124 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5