Detailed information of TA system TA113987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 145491..146086
• Replicon: plasmid p1_BGR15S
• Accession: NZ_CP033663
• Organism: Burkholderia glumae strain BGR15S

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: EFP17_RS02685
• Protein ID: WP_012734107.1
• Coordinates: 145491..145841 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: EFP17_RS02690
• Protein ID: WP_012734108.1
• Coordinates: 145835..146086 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFP17_RS02660 (EFP17_02665)	140713..141459	-	747	WP_232252306.1	energy transducer TonB	-
EFP17_RS02665 (EFP17_02670)	141491..143866	-	2376	WP_232252305.1	TonB-dependent receptor	-
EFP17_RS02670 (EFP17_02675)	144180..144644	-	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
EFP17_RS02675	144867..145088	-	222	WP_017433768.1	hypothetical protein	-
EFP17_RS02680 (EFP17_02680)	145238..145432	-	195	WP_124837685.1	hypothetical protein	-
EFP17_RS02685 (EFP17_02685)	145491..145841	-	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
EFP17_RS02690 (EFP17_02690)	145835..146086	-	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EFP17_RS02695 (EFP17_02695)	146349..146756	-	408	WP_230674618.1	hypothetical protein	-
EFP17_RS02700 (EFP17_02700)	146859..148694	-	1836	WP_012732711.1	site-specific integrase	-
EFP17_RS02705 (EFP17_02705)	148697..149038	-	342	WP_012732710.1	hypothetical protein	-
EFP17_RS02710 (EFP17_02710)	149095..149340	-	246	WP_127913916.1	hypothetical protein	-
EFP17_RS02715	149337..149579	-	243	WP_124837682.1	hypothetical protein	-
EFP17_RS02720 (EFP17_02720)	149593..149877	-	285	WP_017432347.1	hypothetical protein	-
EFP17_RS02725 (EFP17_02725)	150284..150772	-	489	WP_012732893.1	GNAT family N-acetyltransferase	-
EFP17_RS02730 (EFP17_02730)	150769..151038	-	270	WP_012732894.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..153086	153086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T113987 WP_012734107.1 NZ_CP033663:c145841-145491 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T113987 NZ_CP033663:c145841-145491 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT113987 WP_012734108.1 NZ_CP033663:c146086-145835 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT113987 NZ_CP033663:c146086-145835 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5