Detailed information of TA system TA113986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 122008..122645
• Replicon: plasmid p4_BGR15S
• Accession: NZ_CP033662
• Organism: Burkholderia glumae strain BGR15S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: EFP17_RS01895
• Protein ID: WP_012735347.1
• Coordinates: 122008..122409 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: C5AP39
• Locus tag: EFP17_RS01900
• Protein ID: WP_012735348.1
• Coordinates: 122409..122645 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFP17_RS01865 (EFP17_01875)	118471..118740	+	270	WP_012735343.1	hypothetical protein	-
EFP17_RS01870 (EFP17_01880)	118900..119514	+	615	Protein_105	error-prone DNA polymerase	-
EFP17_RS01875 (EFP17_01885)	119530..119817	+	288	WP_012735344.1	hypothetical protein	-
EFP17_RS01880	120921..121115	-	195	WP_124837731.1	hypothetical protein	-
EFP17_RS01885 (EFP17_01895)	121170..121412	-	243	WP_012735345.1	hypothetical protein	-
EFP17_RS01890	121430..121993	-	564	WP_012735346.1	hypothetical protein	-
EFP17_RS01895 (EFP17_01900)	122008..122409	-	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
EFP17_RS01900 (EFP17_01905)	122409..122645	-	237	WP_012735348.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EFP17_RS01905 (EFP17_01915)	122982..123674	-	693	WP_043308722.1	hypothetical protein	-
EFP17_RS01910 (EFP17_01920)	123758..125584	-	1827	WP_012735349.1	site-specific integrase	-
EFP17_RS01915 (EFP17_01925)	125581..125961	-	381	WP_080569442.1	hypothetical protein	-
EFP17_RS01920 (EFP17_01930)	125964..126281	-	318	WP_050811486.1	hypothetical protein	-
EFP17_RS01925 (EFP17_01935)	126332..127420	-	1089	Protein_116	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128913	128913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T113986 WP_012735347.1 NZ_CP033662:c122409-122008 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T113986 NZ_CP033662:c122409-122008 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8983.13 Da
• Isoelectric Point: 6.3676

>AT113986 WP_012735348.1 NZ_CP033662:c122645-122409 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGM

Nucleotide

• Length: 237 bp

>AT113986 NZ_CP033662:c122645-122409 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Structures

Toxin

• AlphaFold DB: C5AP38

Antitoxin

• AlphaFold DB: C5AP39