Detailed information of TA system TA113984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 131235..131830
• Replicon: plasmid p2_BGR13S
• Accession: NZ_CP033658
• Organism: Burkholderia glumae strain BGR13S

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: EFP18_RS29730
• Protein ID: WP_012734107.1
• Coordinates: 131235..131585 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: EFP18_RS29735
• Protein ID: WP_012734108.1
• Coordinates: 131579..131830 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFP18_RS29705 (EFP18_29425)	126457..127203	-	747	WP_232252306.1	energy transducer TonB	-
EFP18_RS29710 (EFP18_29430)	127235..129622	-	2388	WP_257820390.1	TonB-dependent receptor	-
EFP18_RS29715 (EFP18_29435)	129924..130388	-	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
EFP18_RS29720	130611..130832	-	222	WP_017433768.1	hypothetical protein	-
EFP18_RS29725 (EFP18_29440)	130982..131176	-	195	WP_124837685.1	hypothetical protein	-
EFP18_RS29730 (EFP18_29445)	131235..131585	-	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
EFP18_RS29735 (EFP18_29450)	131579..131830	-	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EFP18_RS29740 (EFP18_29455)	132093..132500	-	408	WP_230674618.1	hypothetical protein	-
EFP18_RS29745 (EFP18_29460)	132603..134438	-	1836	WP_012732711.1	site-specific integrase	-
EFP18_RS29750 (EFP18_29465)	134441..134782	-	342	WP_012732710.1	hypothetical protein	-
EFP18_RS29755 (EFP18_29470)	134839..135084	-	246	WP_127913916.1	hypothetical protein	-
EFP18_RS29760	135081..135323	-	243	WP_124837682.1	hypothetical protein	-
EFP18_RS29765 (EFP18_29480)	135337..135621	-	285	WP_017432347.1	hypothetical protein	-
EFP18_RS29770	136014..136211	-	198	WP_124837681.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..136971	136971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T113984 WP_012734107.1 NZ_CP033658:c131585-131235 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T113984 NZ_CP033658:c131585-131235 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT113984 WP_012734108.1 NZ_CP033658:c131830-131579 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT113984 NZ_CP033658:c131830-131579 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5