Detailed information of TA system TA113978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 124865..125460
• Replicon: plasmid p1_YH43
• Accession: NZ_CP033648
• Organism: Burkholderia glumae strain YH43

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: EFO25_RS33175
• Protein ID: WP_012734107.1
• Coordinates: 124865..125215 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: EFO25_RS33180
• Protein ID: WP_012734108.1
• Coordinates: 125209..125460 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFO25_RS33150	120087..120833	-	747	WP_232252306.1	energy transducer TonB	-
EFO25_RS33155	120865..123240	-	2376	WP_232252305.1	TonB-dependent receptor	-
EFO25_RS33160	123554..124018	-	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
EFO25_RS33165	124241..124462	-	222	WP_017433768.1	hypothetical protein	-
EFO25_RS33170	124612..124806	-	195	WP_124837685.1	hypothetical protein	-
EFO25_RS33175	124865..125215	-	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
EFO25_RS33180	125209..125460	-	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EFO25_RS33185	125723..126130	-	408	WP_230674618.1	hypothetical protein	-
EFO25_RS33190	126233..128068	-	1836	WP_012732711.1	site-specific integrase	-
EFO25_RS33195	128071..128412	-	342	WP_012732710.1	hypothetical protein	-
EFO25_RS33200	128469..128714	-	246	WP_127913916.1	hypothetical protein	-
EFO25_RS33205	128711..128953	-	243	WP_124837682.1	hypothetical protein	-
EFO25_RS33210	128967..129251	-	285	WP_017432347.1	hypothetical protein	-
EFO25_RS33215	129639..129956	-	318	WP_148271069.1	hypothetical protein	-
EFO25_RS33220	130140..130421	-	282	WP_043308454.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132944	132944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T113978 WP_012734107.1 NZ_CP033648:c125215-124865 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T113978 NZ_CP033648:c125215-124865 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT113978 WP_012734108.1 NZ_CP033648:c125460-125209 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT113978 NZ_CP033648:c125460-125209 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5