Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 159360..160983 Replicon plasmid pTB-nb4
Accession NZ_CP033636
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag CQP61_RS30825 Protein ID WP_000088046.1
Coordinates 159360..160685 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag CQP61_RS30830 Protein ID WP_024136327.1
Coordinates 160690..160983 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS30800 157090..157530 - 441 WP_072692426.1 recombinase family protein -
CQP61_RS30805 157566..158270 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
CQP61_RS30815 158581..158991 - 411 WP_000985911.1 hypothetical protein -
CQP61_RS30820 159093..159299 - 207 WP_000134171.1 hypothetical protein -
CQP61_RS30825 159360..160685 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
CQP61_RS30830 160690..160983 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
CQP61_RS30840 161587..161979 - 393 WP_000341066.1 hypothetical protein -
CQP61_RS30845 162352..162693 + 342 WP_001151575.1 hypothetical protein -
CQP61_RS30850 162785..162976 + 192 WP_000398480.1 hypothetical protein -
CQP61_RS30855 163026..163307 + 282 WP_000077926.1 hypothetical protein -
CQP61_RS30860 163870..165111 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / lnu(F) / blaTEM-1B / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR - 1..253793 253793


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T113970 WP_000088046.1 NZ_CP033636:c160685-159360 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T113970 NZ_CP033636:c160685-159360 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAATTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCAGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT113970 WP_024136327.1 NZ_CP033636:c160983-160690 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT113970 NZ_CP033636:c160983-160690 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References