Detailed information of TA system TA113969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 5484249..5484892
• Replicon: chromosome
• Accession: NZ_CP033635
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CQP61_RS29620
• Protein ID: WP_049038273.1
• Coordinates: 5484249..5484536 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CQP61_RS29625
• Protein ID: WP_000593555.1
• Coordinates: 5484533..5484892 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS29605	5479266..5480333	+	1068	WP_000361481.1	HlyD family efflux transporter periplasmic adaptor subunit	-
CQP61_RS29610	5480330..5483065	+	2736	WP_000149118.1	ribosome-associated ATPase/putative transporter RbbA	-
CQP61_RS29615	5483065..5484189	+	1125	WP_001314210.1	ABC transporter permease	-
CQP61_RS29620	5484249..5484536	+	288	WP_049038273.1	type II toxin-antitoxin system HicA family toxin	Toxin
CQP61_RS29625	5484533..5484892	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CQP61_RS29630	5484942..5485802	-	861	WP_001131792.1	class II aldolase	-
CQP61_RS29635	5485834..5486103	-	270	WP_000949581.1	HPr family phosphocarrier protein	-
CQP61_RS29640	5486093..5487601	-	1509	WP_012311797.1	carbohydrate kinase	-
CQP61_RS29645	5487594..5488952	-	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
CQP61_RS29650	5489026..5489307	-	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
CQP61_RS29655	5489304..5489777	-	474	WP_012311717.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (34-84)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10490.98 Da
• Isoelectric Point: 7.7845

>T113969 WP_049038273.1 NZ_CP033635:5484249-5484536 [Escherichia coli]
IRTCHENPGTGFTEETKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGA
VESVRDWLLSIGVKP

Nucleotide

• Length: 288 bp

>T113969 NZ_CP033635:5484249-5484536 [Escherichia coli]
ATAAGGACATGCCATGAGAACCCTGGTACAGGCTTTACGGAAGAAACAAAAAATACATTGGATCAAATATTTAAATCTCC
TGTTCCTCAAGGGATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTT
CGCGTTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCT
GTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT113969 WP_000593555.1 NZ_CP033635:5484533-5484892 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT113969 NZ_CP033635:5484533-5484892 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6