Detailed information of TA system TA113968

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5162463..5163295
• Replicon: chromosome
• Accession: NZ_CP033635
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CQP61_RS28150
• Protein ID: WP_096990822.1
• Coordinates: 5162463..5162837 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1E5MC43
• Locus tag: CQP61_RS28155
• Protein ID: WP_063112944.1
• Coordinates: 5162927..5163295 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS28120	5158120..5159043	-	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
CQP61_RS28125	5159154..5160338	-	1185	WP_001172866.1	sugar efflux transporter	-
CQP61_RS28130	5160720..5160866	-	147	WP_001328030.1	hypothetical protein	-
CQP61_RS28135	5161115..5161951	-	837	WP_121832024.1	DUF4942 domain-containing protein	-
CQP61_RS28140	5162041..5162238	-	198	WP_085975623.1	DUF957 domain-containing protein	-
CQP61_RS28145	5162314..5162466	-	153	Protein_4903	hypothetical protein	-
CQP61_RS28150	5162463..5162837	-	375	WP_096990822.1	TA system toxin CbtA family protein	Toxin
CQP61_RS28155	5162927..5163295	-	369	WP_063112944.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CQP61_RS28160	5163345..5163989	-	645	WP_123891640.1	antitoxin of toxin-antitoxin stability system	-
CQP61_RS28165	5164004..5164225	-	222	WP_000692315.1	DUF987 domain-containing protein	-
CQP61_RS28170	5164288..5164764	-	477	WP_072648644.1	RadC family protein	-
CQP61_RS28175	5164780..5165259	-	480	WP_000860074.1	antirestriction protein	-
CQP61_RS28180	5165341..5166159	-	819	WP_001234688.1	DUF945 domain-containing protein	-
CQP61_RS28185	5166345..5167229	-	885	WP_000010380.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5151412..5214876	63464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13977.99 Da
• Isoelectric Point: 7.1881

>T113968 WP_096990822.1 NZ_CP033635:c5162837-5162463 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T113968 NZ_CP033635:c5162837-5162463 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCGGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13724.52 Da
• Isoelectric Point: 6.8270

>AT113968 WP_063112944.1 NZ_CP033635:c5163295-5162927 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT113968 NZ_CP033635:c5163295-5162927 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCACGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E5MC43