Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4555521..4556018 Replicon chromosome
Accession NZ_CP033635
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CQP61_RS25305 Protein ID WP_001173343.1
Coordinates 4555521..4555694 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CQP61_RS25310 Protein ID WP_000398619.1
Coordinates 4555722..4556018 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS25295 4552191..4553648 + 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -
CQP61_RS25300 4553885..4555402 + 1518 WP_000003799.1 lysine--tRNA ligase -
CQP61_RS25305 4555521..4555694 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CQP61_RS25310 4555722..4556018 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CQP61_RS25315 4556246..4556518 - 273 WP_000405647.1 N-acetyltransferase -
CQP61_RS25320 4556530..4556760 - 231 WP_000371713.1 (4Fe-4S)-binding protein -
CQP61_RS25325 4556941..4558572 + 1632 WP_001216471.1 sensor histidine kinase -
CQP61_RS25330 4558569..4559288 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 papB / papI 4481599..4556018 74419


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T113967 WP_001173343.1 NZ_CP033635:c4555694-4555521 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T113967 NZ_CP033635:c4555694-4555521 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT113967 WP_000398619.1 NZ_CP033635:c4556018-4555722 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT113967 NZ_CP033635:c4556018-4555722 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References