Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4369219..4369741 Replicon chromosome
Accession NZ_CP033635
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name relE Uniprot ID V0SDB6
Locus tag CQP61_RS24275 Protein ID WP_001105432.1
Coordinates 4369219..4369509 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag CQP61_RS24280 Protein ID WP_000212715.1
Coordinates 4369499..4369741 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS24260 4364382..4366037 + 1656 WP_012311860.1 alpha,alpha-phosphotrehalase -
CQP61_RS24265 4366431..4368569 + 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
CQP61_RS24270 4368754..4369218 + 465 WP_001009180.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
CQP61_RS24275 4369219..4369509 - 291 WP_001105432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CQP61_RS24280 4369499..4369741 - 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CQP61_RS24290 4369933..4370319 - 387 WP_001232253.1 cytochrome b562 -
CQP61_RS24295 4370375..4371727 - 1353 WP_001162169.1 metalloprotease PmbA -
CQP61_RS24300 4371821..4372372 + 552 WP_000166270.1 ribosome-associated protein -
CQP61_RS24305 4372455..4373828 - 1374 WP_001219785.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11311.21 Da        Isoelectric Point: 10.0238

>T113964 WP_001105432.1 NZ_CP033635:c4369509-4369219 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYSVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T113964 NZ_CP033635:c4369509-4369219 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT113964 WP_000212715.1 NZ_CP033635:c4369741-4369499 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT113964 NZ_CP033635:c4369741-4369499 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SDB6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References