Detailed information of TA system TA113962

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3772471..3773177
• Replicon: chromosome
• Accession: NZ_CP033635
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: V0SXW1
• Locus tag: CQP61_RS21455
• Protein ID: WP_000854677.1
• Coordinates: 3772471..3772839 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: V0SU84
• Locus tag: CQP61_RS21460
• Protein ID: WP_000065824.1
• Coordinates: 3772860..3773177 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS21430	3767851..3769494	+	1644	WP_001265639.1	fimbrial adhesin EcpD	-
CQP61_RS21435	3769463..3770173	+	711	WP_001361803.1	fimbrial chaperone EcpE	-
CQP61_RS21440	3770486..3770815	+	330	WP_001303809.1	YkgJ family cysteine cluster protein	-
CQP61_RS21445	3771064..3771678	-	615	WP_001019920.1	YagU family protein	-
CQP61_RS21455	3772471..3772839	-	369	WP_000854677.1	TA system toxin CbtA family protein	Toxin
CQP61_RS21460	3772860..3773177	-	318	WP_000065824.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CQP61_RS21465	3773197..3773418	-	222	WP_001220307.1	DUF987 domain-containing protein	-
CQP61_RS21470	3773427..3773906	-	480	WP_000438147.1	RadC family protein	-
CQP61_RS21475	3773918..3774376	-	459	WP_000211834.1	antirestriction protein	-
CQP61_RS21480	3774462..3774698	-	237	Protein_3643	DUF905 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3745221..3784163	38942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.89 Da
• Isoelectric Point: 8.2671

>T113962 WP_000854677.1 NZ_CP033635:c3772839-3772471 [Escherichia coli]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T113962 NZ_CP033635:c3772839-3772471 [Escherichia coli]
ATGAAAACTCTACCTGCAACGACTCCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTTAC
TCGTCTACTTGAGCAGCACTATGGCCTAATGTTAAGCGACACACCTTTTAGCGATGAAACAGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTTGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTACGACAGACATTCTTCAAGCTAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11964.46 Da
• Isoelectric Point: 6.3163

>AT113962 WP_000065824.1 NZ_CP033635:c3773177-3772860 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT113962 NZ_CP033635:c3773177-3772860 [Escherichia coli]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACACCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAACT
GCATTATTTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTTACCACTGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
GATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTACTCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: V0SXW1

Antitoxin

• AlphaFold DB: V0SU84