Detailed information of TA system TA113961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3616669..3617506
• Replicon: chromosome
• Accession: NZ_CP033635
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: CQP61_RS20720
• Protein ID: WP_000227787.1
• Coordinates: 3616964..3617506 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: CQP61_RS20715
• Protein ID: WP_001353405.1
• Coordinates: 3616669..3616980 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS20690	3611689..3612636	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
CQP61_RS20695	3612658..3614649	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CQP61_RS20700	3614639..3615253	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CQP61_RS20705	3615253..3615582	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CQP61_RS20710	3615594..3616484	+	891	WP_000971336.1	heme o synthase	-
CQP61_RS20715	3616669..3616980	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
CQP61_RS20720	3616964..3617506	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
CQP61_RS20725	3617562..3618497	-	936	WP_012311701.1	sel1 repeat family protein	-
CQP61_RS20730	3618904..3620268	+	1365	WP_001000978.1	MFS transporter	-
CQP61_RS20735	3620396..3620887	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CQP61_RS20740	3621055..3621966	+	912	WP_000705871.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T113961 WP_000227787.1 NZ_CP033635:3616964-3617506 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T113961 NZ_CP033635:3616964-3617506 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT113961 WP_001353405.1 NZ_CP033635:3616669-3616980 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT113961 NZ_CP033635:3616669-3616980 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGCCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3