Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3582580..3583198 Replicon chromosome
Accession NZ_CP033635
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CQP61_RS20550 Protein ID WP_001291435.1
Coordinates 3582980..3583198 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CQP61_RS20545 Protein ID WP_000344800.1
Coordinates 3582580..3582954 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS20535 3577669..3578862 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CQP61_RS20540 3578885..3582034 + 3150 WP_001132453.1 efflux RND transporter permease AcrB -
CQP61_RS20545 3582580..3582954 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CQP61_RS20550 3582980..3583198 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CQP61_RS20555 3583370..3583921 + 552 WP_000102561.1 maltose O-acetyltransferase -
CQP61_RS20560 3584037..3584507 + 471 WP_000136192.1 YlaC family protein -
CQP61_RS20565 3584671..3586221 + 1551 WP_012311818.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CQP61_RS20570 3586262..3586615 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CQP61_RS20580 3586994..3587305 + 312 WP_000409911.1 MGMT family protein -
CQP61_RS20585 3587336..3587908 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T113960 WP_001291435.1 NZ_CP033635:3582980-3583198 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T113960 NZ_CP033635:3582980-3583198 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT113960 WP_000344800.1 NZ_CP033635:3582580-3582954 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT113960 NZ_CP033635:3582580-3582954 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTCCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References