Detailed information of TA system TA113959

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3250011..3250809
• Replicon: chromosome
• Accession: NZ_CP033635
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0D8WBM4
• Locus tag: CQP61_RS18925
• Protein ID: WP_000847115.1
• Coordinates: 3250435..3250809 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: D8EF63
• Locus tag: CQP61_RS18920
• Protein ID: WP_001285648.1
• Coordinates: 3250011..3250388 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS18885	3246047..3246931	+	885	WP_106509117.1	50S ribosome-binding GTPase	-
CQP61_RS18895	3247117..3247935	+	819	WP_121832183.1	DUF945 domain-containing protein	-
CQP61_RS18900	3248017..3248457	+	441	WP_123891635.1	antirestriction protein	-
CQP61_RS18905	3248512..3248988	+	477	WP_072721126.1	RadC family protein	-
CQP61_RS18910	3249078..3249299	+	222	WP_000706990.1	DUF987 domain-containing protein	-
CQP61_RS18915	3249316..3249960	+	645	WP_000094922.1	hypothetical protein	-
CQP61_RS18920	3250011..3250388	+	378	WP_001285648.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CQP61_RS18925	3250435..3250809	+	375	WP_000847115.1	TA system toxin CbtA family protein	Toxin
CQP61_RS18930	3250806..3251297	+	492	WP_122987109.1	hypothetical protein	-
CQP61_RS18935	3251317..3251514	+	198	WP_001354260.1	DUF957 domain-containing protein	-
CQP61_RS18940	3251610..3252458	+	849	WP_001280451.1	DUF4942 domain-containing protein	-
CQP61_RS18945	3252766..3253425	+	660	WP_001159065.1	glutamine ABC transporter permease GlnP	-
CQP61_RS18950	3253422..3254144	+	723	WP_000569080.1	glutamine ABC transporter ATP-binding protein GlnQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3230747..3250809	20062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14101.21 Da
• Isoelectric Point: 8.1558

>T113959 WP_000847115.1 NZ_CP033635:3250435-3250809 [Escherichia coli]
MKTFPDTHVRATSRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSHYRMVSNITLGKHCEVK

Nucleotide

• Length: 375 bp

>T113959 NZ_CP033635:3250435-3250809 [Escherichia coli]
ATGAAAACATTCCCCGACACACATGTACGGGCAACATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGTGCCTGCACCCGCTCTCAGTTAATCAACAGCATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACCGAATGGTGAGCAACATCACCCTGGGTAAACACTGTGAGGTGAAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13685.41 Da
• Isoelectric Point: 5.9505

>AT113959 WP_001285648.1 NZ_CP033635:3250011-3250388 [Escherichia coli]
MSDTLTGTTHPDDNSDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYIAIYPTPATPGTTA

Nucleotide

• Length: 378 bp

>AT113959 NZ_CP033635:3250011-3250388 [Escherichia coli]
ATGTCAGACACACTCACCGGGACAACGCATCCCGACGACAACAGCGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGCATCAGAGGCCGGT
TCAGCGACGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAGCTGGAACTCATGCTCACCAGTGGT
GAACTCAGTCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTGTATATCGCCATTTATCCGACACCGGCAACACCCGGAACCACCGCATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WBM4

Antitoxin

• AlphaFold DB: A0A1M2EN88