Detailed information of TA system TA113958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3195659..3196364
• Replicon: chromosome
• Accession: NZ_CP033635
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CQP61_RS18620
• Protein ID: WP_000539521.1
• Coordinates: 3195659..3196045 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CQP61_RS18625
• Protein ID: WP_001280945.1
• Coordinates: 3196035..3196364 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS18600	3191663..3192289	+	627	WP_001295292.1	glutathione S-transferase GstB	-
CQP61_RS18605	3192286..3193401	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
CQP61_RS18610	3193512..3193895	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CQP61_RS18615	3194108..3195433	+	1326	WP_121832019.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CQP61_RS18620	3195659..3196045	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CQP61_RS18625	3196035..3196364	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CQP61_RS18630	3196434..3197762	-	1329	WP_000086869.1	GGDEF domain-containing protein	-
CQP61_RS18635	3197770..3200118	-	2349	WP_000950337.1	EAL domain-containing protein	-
CQP61_RS18640	3200295..3201206	-	912	WP_001236058.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T113958 WP_000539521.1 NZ_CP033635:3195659-3196045 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T113958 NZ_CP033635:3195659-3196045 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT113958 WP_001280945.1 NZ_CP033635:3196035-3196364 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT113958 NZ_CP033635:3196035-3196364 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6