Detailed information of TA system TA113931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 94390..94915
• Replicon: plasmid pTB-nb2
• Accession: NZ_CP033633
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: CQP61_RS01655
• Protein ID: WP_001159871.1
• Coordinates: 94610..94915 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: CQP61_RS01650
• Protein ID: WP_121832161.1
• Coordinates: 94390..94608 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS01610	89751..89981	+	231	WP_001261278.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CQP61_RS01615	89978..90394	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-
CQP61_RS01625	90956..92169	+	1214	WP_164473236.1	IS3 family transposase	-
CQP61_RS01630	92560..93012	+	453	WP_032353631.1	N-acetyltransferase	-
CQP61_RS01635	93044..93229	-	186	WP_032353630.1	hypothetical protein	-
CQP61_RS01640	93277..93408	-	132	Protein_107	transposase	-
CQP61_RS01650	94390..94608	+	219	WP_121832161.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CQP61_RS01655	94610..94915	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
CQP61_RS01660	94916..95725	+	810	WP_045145707.1	site-specific integrase	-
CQP61_RS01665	95863..96560	+	698	Protein_111	IS1 family transposase	-
CQP61_RS01670	96577..97455	-	879	Protein_112	colicin-B	-
CQP61_RS01675	97632..98129	+	498	WP_072157241.1	tyrosine-type recombinase/integrase	-
CQP61_RS01680	98266..98541	-	276	WP_001536593.1	hypothetical protein	-
CQP61_RS01685	98535..99179	-	645	WP_000633911.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..139752	139752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T113931 WP_001159871.1 NZ_CP033633:94610-94915 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T113931 NZ_CP033633:94610-94915 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8388.28 Da
• Isoelectric Point: 4.5203

>AT113931 WP_121832161.1 NZ_CP033633:94390-94608 [Escherichia coli]
MKQRITVTVDSDSYQFLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT113931 NZ_CP033633:94390-94608 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTTCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8