Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 89751..90394 Replicon plasmid pTB-nb2
Accession NZ_CP033633
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag CQP61_RS01615 Protein ID WP_001034046.1
Coordinates 89978..90394 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag CQP61_RS01610 Protein ID WP_001261278.1
Coordinates 89751..89981 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS01580 84893..85072 + 180 WP_077250632.1 Hin recombinase -
CQP61_RS01590 85332..86195 - 864 WP_021534910.1 endonuclease/exonuclease/phosphatase family protein -
CQP61_RS01595 86377..87074 - 698 Protein_99 IS1 family transposase -
CQP61_RS01600 87305..87694 - 390 WP_045146805.1 cytochrome B562 -
CQP61_RS01605 88079..89566 - 1488 Protein_101 IncI1-type relaxase NikB -
CQP61_RS01610 89751..89981 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CQP61_RS01615 89978..90394 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
CQP61_RS01625 90956..92169 + 1214 WP_164473236.1 IS3 family transposase -
CQP61_RS01630 92560..93012 + 453 WP_032353631.1 N-acetyltransferase -
CQP61_RS01635 93044..93229 - 186 WP_032353630.1 hypothetical protein -
CQP61_RS01640 93277..93408 - 132 Protein_107 transposase -
CQP61_RS01650 94390..94608 + 219 WP_121832161.1 type II toxin-antitoxin system antitoxin CcdA -
CQP61_RS01655 94610..94915 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139752 139752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T113930 WP_001034046.1 NZ_CP033633:89978-90394 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T113930 NZ_CP033633:89978-90394 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATAGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT113930 WP_001261278.1 NZ_CP033633:89751-89981 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT113930 NZ_CP033633:89751-89981 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References