Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 77811..78370 Replicon plasmid pTB-nb2
Accession NZ_CP033633
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag CQP61_RS01525 Protein ID WP_000421257.1
Coordinates 77811..78086 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0H2XL20
Locus tag CQP61_RS01530 Protein ID WP_012006537.1
Coordinates 78086..78370 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS01500 73115..74473 + 1359 WP_032353707.1 esterase-like activity of phytase family protein -
CQP61_RS01505 74527..75253 - 727 Protein_83 IS256 family transposase -
CQP61_RS01510 75361..75741 + 381 WP_001171523.1 IS66 family insertion sequence hypothetical protein -
CQP61_RS01515 75738..76085 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
CQP61_RS01520 76135..77672 + 1538 Protein_86 IS66-like element ISEc8 family transposase -
CQP61_RS01525 77811..78086 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CQP61_RS01530 78086..78370 - 285 WP_012006537.1 ribbon-helix-helix domain-containing protein Antitoxin
CQP61_RS01540 78871..79461 + 591 WP_000086536.1 recombinase family protein -
CQP61_RS01545 79508..79666 + 159 Protein_90 integrase core domain-containing protein -
CQP61_RS01550 79816..80814 + 999 WP_032353474.1 ABC transporter substrate-binding protein -
CQP61_RS01555 80814..81851 + 1038 WP_032353476.1 iron ABC transporter permease -
CQP61_RS01560 81851..82612 + 762 WP_000020503.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139752 139752
- inside IScluster/Tn - - 69287..79461 10174


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T113929 WP_000421257.1 NZ_CP033633:c78086-77811 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T113929 NZ_CP033633:c78086-77811 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10501.96 Da        Isoelectric Point: 6.4632

>AT113929 WP_012006537.1 NZ_CP033633:c78370-78086 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT113929 NZ_CP033633:c78370-78086 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCAATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGAAATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2XL20

References