Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 53505..54040 Replicon plasmid pTB-nb2
Accession NZ_CP033633
Organism Escherichia coli isolate ECCNB12-2

Toxin (Protein)


Gene name relE Uniprot ID A0A0D8WEY8
Locus tag CQP61_RS01375 Protein ID WP_032353555.1
Coordinates 53753..54040 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0D8WHE0
Locus tag CQP61_RS01370 Protein ID WP_045145550.1
Coordinates 53505..53756 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQP61_RS01330 48651..49271 - 621 WP_032353888.1 DUF1449 family protein -
CQP61_RS01340 49538..50203 - 666 WP_021547664.1 hypothetical protein -
CQP61_RS01345 50344..50985 - 642 WP_021547665.1 hypothetical protein -
CQP61_RS01355 51686..52558 + 873 WP_045145554.1 incFII family plasmid replication initiator RepA -
CQP61_RS31340 53251..53418 + 168 WP_045145553.1 hypothetical protein -
CQP61_RS01370 53505..53756 + 252 WP_045145550.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CQP61_RS01375 53753..54040 + 288 WP_032353555.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CQP61_RS31345 54050..54337 + 288 WP_045145547.1 hypothetical protein -
CQP61_RS01390 55498..55944 + 447 WP_021560313.1 serine acetyltransferase -
CQP61_RS01395 56224..56817 - 594 WP_032353553.1 proQ/FINO family protein -
CQP61_RS01415 57671..58859 - 1189 Protein_66 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139752 139752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11114.16 Da        Isoelectric Point: 10.6261

>T113928 WP_032353555.1 NZ_CP033633:53753-54040 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T113928 NZ_CP033633:53753-54040 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCACTGAAAGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTAAAAAAATGCAGTGAAAATCCTCATATAGCCTCTGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9219.48 Da        Isoelectric Point: 4.1789

>AT113928 WP_045145550.1 NZ_CP033633:53505-53756 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHYVDLD
SYL

Download         Length: 252 bp

>AT113928 NZ_CP033633:53505-53756 [Escherichia coli]
ATGCCCAGTATAATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGGATGC
TTGATGCCCTGGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAGTCAGCCTCTGCATTATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WEY8

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8WHE0

References