Detailed information of TA system TA113927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 120194..120795
• Replicon: plasmid pTB-nb1
• Accession: NZ_CP033632
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: CQP61_RS00825
• Protein ID: WP_001216045.1
• Coordinates: 120415..120795 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CQP61_RS00820
• Protein ID: WP_001190712.1
• Coordinates: 120194..120415 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS00765	115286..115546	+	261	WP_000969524.1	hypothetical protein	-
CQP61_RS00770	115546..115827	+	282	WP_000002126.1	ASCH domain-containing protein	-
CQP61_RS00775	115851..116144	+	294	WP_000267998.1	hypothetical protein	-
CQP61_RS00780	116151..116525	+	375	WP_000988652.1	hypothetical protein	-
CQP61_RS00785	116507..117181	+	675	WP_032236592.1	hypothetical protein	-
CQP61_RS00790	117178..117540	+	363	WP_001261543.1	hypothetical protein	-
CQP61_RS00805	118616..118972	-	357	WP_001062545.1	hypothetical protein	-
CQP61_RS00810	118973..119557	-	585	WP_023356284.1	hypothetical protein	-
CQP61_RS00815	119732..120121	+	390	WP_104774974.1	DNA repair protein	-
CQP61_RS00820	120194..120415	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CQP61_RS00825	120415..120795	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CQP61_RS00830	120800..120979	+	180	WP_001339207.1	hypothetical protein	-
CQP61_RS00835	121007..122050	+	1044	WP_023356283.1	DUF968 domain-containing protein	-
CQP61_RS00840	122139..122591	+	453	WP_001326849.1	late promoter-activating protein	-
CQP61_RS00845	122677..123870	+	1194	WP_000219625.1	hypothetical protein	-
CQP61_RS00850	123870..125354	+	1485	WP_000124150.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	qacE / ant(3'')-Ia / dfrA1 / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / mph(A) / aph(3')-IIa / oqxB / oqxA / blaCTX-M-65 / fosA3 / tet(A)	-	1..147451	147451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T113927 WP_001216045.1 NZ_CP033632:120415-120795 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T113927 NZ_CP033632:120415-120795 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCTGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT113927 WP_001190712.1 NZ_CP033632:120194-120415 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT113927 NZ_CP033632:120194-120415 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6