Detailed information of TA system TA113926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 88951..89473
• Replicon: plasmid pTB-nb1
• Accession: NZ_CP033632
• Organism: Escherichia coli isolate ECCNB12-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: CQP61_RS00580
• Protein ID: WP_000220561.1
• Coordinates: 89192..89473 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: CQP61_RS00575
• Protein ID: WP_000121743.1
• Coordinates: 88951..89202 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQP61_RS00530	84112..84321	-	210	Protein_91	IS1 family transposase	-
CQP61_RS00535	84386..85090	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
CQP61_RS00545	85158..85742	+	585	WP_001137892.1	TetR family transcriptional regulator	-
CQP61_RS00560	86235..86999	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
CQP61_RS00570	87140..87844	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
CQP61_RS00575	88951..89202	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
CQP61_RS00580	89192..89473	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CQP61_RS31315	89712..89804	-	93	Protein_98	IS6 family transposase	-
CQP61_RS00590	89970..91169	-	1200	WP_000948429.1	IS91 family transposase	-
CQP61_RS00595	91179..91367	-	189	WP_000957857.1	hypothetical protein	-
CQP61_RS00600	91487..92917	+	1431	Protein_101	IS4-like element IS50R family transposase	-
CQP61_RS00605	92946..93740	+	795	WP_000572405.1	aminoglycoside O-phosphotransferase APH(3')-IIa	-
CQP61_RS00610	93761..93997	+	237	Protein_103	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	qacE / ant(3'')-Ia / dfrA1 / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / mph(A) / aph(3')-IIa / oqxB / oqxA / blaCTX-M-65 / fosA3 / tet(A)	-	1..147451	147451
	-	inside	IScluster/Tn	aac(3)-IId / mph(A) / aph(3')-IIa / oqxB / oqxA / blaCTX-M-65 / fosA3	-	73148..111316	38168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T113926 WP_000220561.1 NZ_CP033632:89192-89473 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T113926 NZ_CP033632:89192-89473 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT113926 WP_000121743.1 NZ_CP033632:88951-89202 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT113926 NZ_CP033632:88951-89202 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301