Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5611223..5611848 Replicon chromosome
Accession NZ_CP033631
Organism Klebsiella sp. P1CD1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag DTA24_RS27720 Protein ID WP_008807903.1
Coordinates 5611223..5611606 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DTA24_RS27725 Protein ID WP_004150355.1
Coordinates 5611606..5611848 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTA24_RS27705 5608590..5609492 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DTA24_RS27710 5609489..5610124 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
DTA24_RS27715 5610121..5611050 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DTA24_RS27720 5611223..5611606 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
DTA24_RS27725 5611606..5611848 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
DTA24_RS27730 5612053..5612970 + 918 WP_110196585.1 alpha/beta hydrolase -
DTA24_RS27735 5612985..5613926 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
DTA24_RS27740 5613971..5614408 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
DTA24_RS27745 5614405..5615265 - 861 WP_008807907.1 virulence factor BrkB family protein -
DTA24_RS27750 5615259..5615858 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T113924 WP_008807903.1 NZ_CP033631:c5611606-5611223 [Klebsiella sp. P1CD1]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T113924 NZ_CP033631:c5611606-5611223 [Klebsiella sp. P1CD1]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT113924 WP_004150355.1 NZ_CP033631:c5611848-5611606 [Klebsiella sp. P1CD1]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT113924 NZ_CP033631:c5611848-5611606 [Klebsiella sp. P1CD1]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References